中国实用神经疾病杂志2017，Vol.20Issue(24)：13-17,5.DOI:10.3969/j.issn.1673-5110.2017.24.003
尿酸在脑梗死中的神经保护作用机制研究
Neuroprotective mechanism of uric acid in cerebral infarction
摘要
Abstract
Objective To explore the specific mechanism of uric acid in cerebral infarction .Methods Oxygen glucose depri-vation (OGD) model was constructed using HT22 cells ,and then the cells were stimulated with different concentrations of uric acid .The cell proliferation was detected by MTT ,the apoptosis was detected by TUNEL method ,and the production of ROS was detected at the same time .To establish the model of middle cerebral artery occlusion (MCAO) by using thread embolism method , and treated them with the appropriate concentration of uric acid to detect the brain tissue defect .Results Uric acid below 450μM did not have significant effect on proliferation of cells ,but 1000μmol/L uric acid significantly inhibited proliferation of cells .Uric acid at a low concentration (50μmol/L) improved proliferation and apoptosis of cells ,and ROS levels during OGD/reoxygenation ;and a suitable concentration (300 μmol/L ) of uric acid significantly improved proliferation and apoptosis of cells ,and reduce ROS production during OGD/reoxygenation ;but a high concentration (1000 μmol/L) of uric acid further reduced proliferation of cells and enhance ROS production .At the same time ,uric acid significantly reduced the level of brain tissue damage in MCAO model rats .Conclusion Therefore ,different concentrations of uric acid have different effect ,and suitable concentrations of uric acid have neuroprotective effects .This finding may provide guidance for study of the clinical value of uric acid .关键词
尿酸/脑梗死/神经保护作用/细胞增殖/大鼠Key words
Uric acid/Cerebral infarction/Protective effect/Cell proliferation/Rat分类
医药卫生引用本文复制引用
莫志铭..尿酸在脑梗死中的神经保护作用机制研究[J].中国实用神经疾病杂志,2017,20(24):13-17,5.基金项目
2016 年广东省科技发展专项资金项目 ,编号 :2016A030313609 （）
2016 年广东省医学科研基金项目 ,编号 :A2016199 （）
2016年广东省教育厅特色创新项目(自然科学) ,编号 :2016KTSCX112 （自然科学）
