中医药导报2017，Vol.23Issue(23)：26-29,53,5.
新疆阿魏乙酸乙酯部位对人结肠癌Caco-2细胞周期和凋亡的影响术
Effects of Ferula Sinkiangensis K.M.Shen Ethyl Acetate Parts on the Cell Cycle and Apoptosis of Human Colon Cancer Caco-2 Cells
摘要
Abstract
Objective:To study the effect of Ferula sinkiangensis K.M.Shen optimization ethyl acetate parts on colon Caco-2 and the influence of cell cycle and apoptosis of tunor suppressor gene PTEN,p53.Methods:The Caco-2 cells were intervened by Ferula sinkiangensis K.M.Shen ethyl acetate parts with complete nutrient solution with the concentration of 15,30,60 μg/nL solution respectively.Apoptosis for each cell cycle were detected by flow eytometry instrument.Apoptosis and cell cycle related tumor suppressor gene PTEN,the relative expression level of p53 gene and protein were detected by rt-pcr and Western Bloting detection.Results:A large number of Caco-2 cells were in G0/G1 phase in Ferula sinkiangensis K.M.Shen ethyl acetate group (P＜0.05),and medicine group showed higher Caco-2 cells apoptosis than the blank group (P＜0.01);Rt-pcr and Western Bloting testing results showed that Caco-2 cells PTEN and p53 gene expression levels were significantly increased in the medicine group (P＜0.01),and that the relative expression level of PTEN and p53 protein is also significantly increased (P＜0.05 or P＜0.01);the effects were similar with the positive drug cisplatin.Conclusion:Ferula sinkiangensis K.M.Shen ethyl acetate parts can block the Caco-2 cell division of human colon cancer in G0/G1 phase,promote Caco-2 cell apoptosis,and increase the expression of tumor suppressor gene PTEN and p53 gene has important link..关键词
新疆阿魏/周期/凋亡/PTEN基因/p53基因/Caco-2细胞Key words
Ferula sinkiangensis K.M.Shen/cycle/apoptosis/PTEN gene/p53 gene/Caco-2 cel分类
医药卫生引用本文复制引用
刘柏里,王纤汝,王冲,郭萍萍,张海英..新疆阿魏乙酸乙酯部位对人结肠癌Caco-2细胞周期和凋亡的影响术[J].中医药导报,2017,23(23):26-29,53,5.基金项目
国家自然科学基金项目(81260625,81660657) （81260625,81660657）
