基础医学与临床2018，Vol.38Issue(1)：7-12,6.
肥胖抑制素减轻高糖致大鼠INS1细胞的凋亡
Obestatin attenuates apoptosis induced by high glucose in INS 1 cells
摘要
Abstract
Objective To investigate the effect of obestatin on the apoptosis of rat pancreatic islet cell line INS1 induced by high glucose .Methods INS1 cells were cultured in different concentrations of glucose .The survival rate and proliferation of INS1 cells were detected by MTT method;Hoechst33258 nuclear staining was used to de-tect nuclear morphology.caspase-3 method was used to study the relationship between the protective effect of obestatin and the PI3K pathway;Finally,using real-time PCR detection of FOXO1 and SREBP1c, Bax, PDX-1 expression, to further clarify the protective effect of obestatin on cells.Results In high glucose condition,obesta-tin promoted the proliferation of INS1 cells at 100 nmol/L,and promoted the proliferation of INS 1 cells significantly ( P<0.01 , compared with the control group and high glucose group ) .Obestatin can reduce high glucose-induced apoptosis(P<0.01).The expressions of FOXO1,SREBP1c,Bax and PDX-1 decreased,while the expression of FOXO1,SREBP1c,Bax and PDX-1 increased in high glucose group .Conclusions OB can attenuate the injury of INS1 cells induced by high glucose in rats .关键词
肥胖抑制素/糖毒性/INS1细胞/保护作用Key words
obestatin/glucotoxicity/INS1 cells/protection分类
社会科学引用本文复制引用
徐敏,王威,姜方旭,乔虹,张玲..肥胖抑制素减轻高糖致大鼠INS1细胞的凋亡[J].基础医学与临床,2018,38(1):7-12,6.基金项目
国家自然科学基金(81471081) （81471081）
黑龙江省自然科学基金(H201416) （H201416）
黑龙江省教育厅科学技术研究项目(11551199) （11551199）
哈尔滨医科大学附属第二医院博士基金(BS2011-12) （BS2011-12）
