基础医学与临床2018，Vol.38Issue(1)：13-19,7.
Akt在Islet-1诱导间充质干细胞C3H10T1/2向心肌细胞特异分化过程中的作用
Role of Akt during Islet-1 inducing C3H10T1/2 cells to differentiate specifically into cardiomyocytes
摘要
Abstract
Objective To study the effect and mechanism of Akt during Islet -1 inducing the mesenchymal stem cells of C3H10T 1/2 cells to differentiate specifically into cardiomyocytes.Methods Developing a model of Islet-1 over-expression cells, Lentivirus infection efficiency of cells was detected by flow cytometry detection tech-nology.Cell proliferation was tested by CCK-8.The protein expression of Islet-1, cTnT and p-Akt/T-Akt was measured by Western blot.The mRNA expression of GATA4,Nkx2.5 and Mef2c were tested by real-time PCR. Results With the increasing of MK-2206 concentration, the inhibition rate of cell proliferation increased ( P<0.05) ,and the best inhibition concentration of Akt was 8nmol /L; With prolongation of Islet-1 inducing time,the protein expression of p-Akt/TA-kt reduced ( P<0.05 );The gene expression of myocardial specific transcription factor GATA4, Nkx2.5 andMe f2c increased( P<0.05); treated with MK-2206, the geneexpres-sion of GATA4, Nkx2.5 and Mef2cincreased significantly at the first week and then reduced ( P<0.05) . Conclu isons Akt plays different effects in different differentiation stages during the Islet-1 inducing the cells into cardiacs-pecific differentiation process .关键词
Islet1-/Akt/MK-2206/间充质干细胞/心肌细胞Key words
Islet (1-)/Akt/MK-2206/mesenchymal stem cells/cardiomyocyte分类
医药卫生引用本文复制引用
赖星宇,朱静,田杰,易勤,陈雪妮..Akt在Islet-1诱导间充质干细胞C3H10T1/2向心肌细胞特异分化过程中的作用[J].基础医学与临床,2018,38(1):13-19,7.基金项目
国家自然科学基金(81670270) （81670270）
