激光生物学报2017，Vol.26Issue(6)：565-569,5.DOI:10.3969/j.issn.1007-7146.2017.06.015
猪RegIIIγ在小肠表达的特征及细胞类型
Expression Patterns and Cell Type of Porcine RegIIIγ in Small Intestine
摘要
Abstract
RegIIIγ, a kind of novel antimicrobial protein with multiple functions, such as antibacterial, anti-inflamma-tion, modulation of immune responses, and stimulation of cell proliferation, is highly expressed in intestine and skin of mammalian. To investigate the expression pattern and cell type, the mRNA and protein expression levels of RegIIIγ in neonatal piglets' intestine from 0-5 weeks were analyzed by real-time PCR and western bolt, and cells expressing RegIIIγ were determined by immunohistochemistry. PCR and western blot analysis showed that there were no significant differences of RegIIIγmRNA and protein among the duodenum, jejunum, and ileum, and the mRNA and protein levels exhibit an increase trend with the development except for a decrease of mRNA after weaning. Immunohistochemistry assay revealed that RegIIIγ mainly expressed in absorption enterocytes and Paneth cells.关键词
RegIIIγ/荧光定量PCR/蛋白质免疫印迹/免疫组化Key words
RegIIIγ/real-time PCR/western blot/immunohistochemistry分类
医药卫生引用本文复制引用
林梦雨,吴洁,杜霞,张婉莹,李琳,陈韬..猪RegIIIγ在小肠表达的特征及细胞类型[J].激光生物学报,2017,26(6):565-569,5.基金项目
湖南省大学生创新实验项目(SCX1608) （SCX1608）
