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一株可降解聚己内酯的细菌菌株选育及其生物降解过程

于丹 付晓 李凡 陈珊

吉林农业大学学报2017,Vol.39Issue(5):539-543,5.
吉林农业大学学报2017,Vol.39Issue(5):539-543,5.DOI:10.13327/j.jjlau.2017.3834

一株可降解聚己内酯的细菌菌株选育及其生物降解过程

Breeding and Biodegradation of A PCL Degradable Bacterial Strain

于丹 1付晓 2李凡 3陈珊3

作者信息

  • 1. 长春医学高等专科学校,长春130031
  • 2. 华大基因,天津300308
  • 3. 东北师范大学生命科学学院,长春130024
  • 折叠

摘要

Abstract

A strain with poly (ε-caprolactone) (PCL) degradable ability was isolated from activated sludge which was achieved from sewage disposal plant in the brewery.The strain was identified as Pseudomonas sp.by colony morphology,physiological and 16S rDNA analysis.The PCL film was degraded completely within 72 h under the condition of liquid culture.The surface structure of PCL films which had been degraded for different days was observed with SEM,and it showed that PCL degradation occurred first in the surface of PCL and then in the deeper area.The main degraded products of the strain were identified as PCL monomer and two polymers by mass spectrometric analysis.

关键词

聚己内酯/生物降解/菌株选育/假单胞菌属

Key words

poly-caprolactone(PCL)/biodegradation/strain breeding/Pseudomonas sp.

分类

资源环境

引用本文复制引用

于丹,付晓,李凡,陈珊..一株可降解聚己内酯的细菌菌株选育及其生物降解过程[J].吉林农业大学学报,2017,39(5):539-543,5.

基金项目

吉林省科技发展计划项目(20150520032JH),吉林省教育厅项目(吉教科合字2015第459号) (20150520032JH)

吉林农业大学学报

OA北大核心CSCDCSTPCD

1000-5684

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