摘要
Abstract
Objective To investigate the diagnosis value of clinical manifestations、cytologic examination、immunophenotype and cytogenetic features of chronic lymphocytic leukemia (CLL).Methods Clinical records and laboratory features of 38 patients from our institution with CLL diagnosed were retrospectively reviewed.Results Most of the patients had typical clinical manifestations when newly diagnosed:77.3% cases with lymphadenectasis,59% cases with splenomegaly,43% cases with anaemia while thrombo-cytopenia、hepatomegaly and fever are not common.The mean of WBC of peripheral blood is 68.3×109/L,of lymphocyte count is 58.9×109/L and of lymphocyte proportion is 84.5%.The levels of LDH:135~550U/L,22.% cases were supernormal.The re-sults of immunophenotype indicated that among 38 cases the positive rates of CD19 was 100%,followed by HLA-DR (97.3% ), CD79a (93.5% ),CD20(89.5% ),CD23(78.4% ),CD5(78.4% ),while CD22、cIgm slightly expressed.All cases were divided into two groups by CD5+ and CD5-.There was not significant difference in the expression of the major antigens of B-cell CLL between the two groups(P>0.05).7 cases were analysed by fluorescence in situ hybridization (FISH).We find 6 kinds of aberrations in a-bove cases:13q14.3 deletion,13q14.3 deletion,trisomy 12,t(14,16)(q32;q21.3),CSP12 amplification、IGH abnormality.The most frequent abnormality was 13q14.3 deletion,which was detected in 3 (42.9% )patients,followed 13q14.3 deletion in 2 (28.6% )pa-tients.6 patients (85.7% )had at least one aberration.In particular,3 (42.9% )patients had a single abnormality and 2(28.6% ) patients had two abnormalities and 1 (14.3% )patient had three abnormalities.Conclusion Immunophenotyping and cytogenetic features play a significant role in the diagnosis and prognosis of CLL.However,they have restriction of specificity.Clinical manifes-tations and cytogenetic features have assistant diagnostic value.We should combine typical clinical manifestations,the cell morphol-ogy,immunophenotype and molecular genetics detection for accurate diagnosis.关键词
慢性淋巴细胞白血病/免疫表型/回顾性研究Key words
chronic lymphocytic leukemia/immunophenotype/retrospective analysis
