林产化学与工业2017，Vol.37Issue(6)：65-72,8.DOI:10.3969/j.issn.0253-2417.2017.06.009
缅甸大果紫檀精油的化学成分及其抑菌活性研究
Chemical Component and Antibacterial Activity of Essential Oil from Myanmar Pterocarpus macrocarpus
摘要
Abstract
The essential oils of Myanmar Pterocarpus macrocarpus Kurz were obtained by using steam distillation(SD), solvent thermal extraction(STE) and subcritical fluid extraction(SFE), with the comparative analysis of the chemical composition of three methods. The results showed that SFE exhibited the highest essential oil yield of 1. 95%, SD exhibited the lowest essential oil yield of 1. 03%, the yield of STE was 1. 74%. About 38 constituents of the essential oil obtained by SD were identified by GC-MS analysis with the main compounds of β-eudesmol ( 69. 897%) and machilol ( 18. 055%) . About 48 constituents of the essential oil obtained by STE were identified with the main compounds of β-eudesmol ( 50. 907%) , homopterocarpin (13. 322%), machilol(9. 333%), 1-(1,1-dimethylethyl)-4-methoxy-benzene(2. 481%) and espatulenol(2. 285%). About 48 constituents of the essential oil obtained by SFE were identified with the main compounds of β-eudesmol ( 47. 786%) , homopterocarpin(13. 516%), dimethyl 2,3-dicyano-2-butenedioate(5. 429%), machilol(5. 232%), 1-(1,1-dimethylethyl)-4-methoxy-benzene( 2. 699%) and espatulenol ( 2. 636%) . The antimicrobial activity of the essential oils obtained by threee extraction methods were tested by zone of inhibition test, MIC and MBC assays. The essential oil obtained by SD showed no antimicrobial activity against Klebsiella pneumoniae, Candida albicans and Bacillus subtilis. However, it showed higher antimicrobial activity with the MIC values against the Klebsiella pneumoniae, Candida albicans and Bacillus subtilis of 6. 25, 25 and 6. 25 g/L, respectively.关键词
大果紫檀/精油/水蒸气蒸馏/亚临界流体萃取/抑菌活性Key words
Pterocarpus macrocarpus Kurz/essential oil/steam distillation/subcritical fluid extraction/antibacterial activity分类
化学化工引用本文复制引用
陈虹霞,康秀棠,尤龙杰,王成章,叶建中..缅甸大果紫檀精油的化学成分及其抑菌活性研究[J].林产化学与工业,2017,37(6):65-72,8.基金项目
国家国际科技合作专项(2014DFR31300) （2014DFR31300）
