农业环境科学学报2017，Vol.36Issue(12)：2379-2387,9.DOI:10.11654/jaes.2017-0764
MC-LR急性胁迫对草鱼脾脏、头肾显微结构及BAFF和APRIL基因表达的影响
Acute effects of microcystin-LR on the microstructure of spleen and head kidney and the expression of BAFF and APRIL genes in grass carp
摘要
Abstract
In order to investigate the immunotoxicity of microcystin-LR(MC-LR)in grass carp, an acute toxicity test was used. The grass carp individuals were injected intraperitoneally with different doses of MC-LR, and the histopathologies of their spleens and head kidneys were evaluated after 24, 48, 72, and 96 h. The results showed that cell vacuolization occurred and the number of melano-macrophages first increased and then decreased, especially in the 75-and 100-μg MC-LR·kg-1 groups at 48 h, and the volume of melano-macrophage center was significantly increased. The sinusoid broadening, lymphocyte disordering, and angiectasis were mainly observed in the head kidney. In the 75-and 100-μg MC-LR·kg-1 groups, the lymphoid tissues were loosely and disorderly arranged, showing the vacuolization and even dissolution of some lymphocytes. Furthermore, a quantitative RT-PCR was applied to detect the expression of the BAFF and APRIL genes in the spleen and head kidney of the grass carp individuals exposed to MC-LR for 96 h. The expressions of both BAFF and APRIL genes were downregulated to different extents. The BAFF gene was significantly inhibited in the spleen of the 75-μg MC-LR·kg-1 group individ-uals(P<0.05), and it was significantly inhibited in the head kidney of the 75- and 100-μg MC-LR·kg-1 group individuals. Moreover, the APRIL gene was significantly inhibited in the spleen and head kidney of all groups, except for the spleen of the 25-μg MC-LR·kg-1 group individuals (P<0.05). This study suggested that MC-LR could damage the immune organs and inhibit the genes related to B-lym-phocyte proliferation in a time-and dose-dependent manner. The above-mentioned histological damage and gene expression changes indi-cate that MC-LR reduces the immune function of fish.关键词
微囊藻毒素-LR/草鱼/脾脏/头肾/组织病理/BAFF/APRILKey words
microcystin-LR/grass carp/spleen/head kidney/histopathology/BAFF/APRIL分类
资源环境引用本文复制引用
隗黎丽,刘毅,王自蕊,阮记明,周颖,熊六凤,钟其旺..MC-LR急性胁迫对草鱼脾脏、头肾显微结构及BAFF和APRIL基因表达的影响[J].农业环境科学学报,2017,36(12):2379-2387,9.基金项目
国家自然科学基金项目(31460146,31260643,31760764) The National Natural Science Foundation of China(31460146,31260643,31760764) （31460146,31260643,31760764）
