农业生物技术学报2017，Vol.25Issue(12)：1970-1978,9.DOI:10.3969/j.issn.1674-7968.2017.12.007
盐和干旱胁迫下红麻HcWD40-1基因的克隆及表达特征
Cloning and Expression Characteristics of HcWD40-1 Gene Under Salt and Drought Stress in Kenaf(Hibiscus cannabinus)
摘要
Abstract
WD-repeat protein,containing WD-40 motif,also known as WD40 repeat protein,which plays an essential regulating role during the abiotic stress response of plants.In this study,it was found that a kenaf (Hibiscus cannabinus) transcriptome unigene which was highly similar to the WD40 gene,and then designed the primers to carry out reverse transcription PCR amplification and obtained HcWD40-1(GenBank No.:KX711617) cDNA sequence by sanger sequencing.Bioinformatics analysis showed that the gene open reading frame was 1 356 bp,encoding 451 amino acids,and containing 7 typical WD40 domains,qRT-PCR analysis showed that HcWD40-1 gene was induced by salt and drought stress,and was induced by abscisic acid (ABA) and methyl jasmonate (MeJA).The results suggested that the HcWD40-1 gene was a key hub gene for ABA and MeJA signaling pathways,salt and drought stress responses.The results of the study provide basic data for the study of salt-tolerant,drought-resistant on ABA signal regulation network and MeJA signal regulation network of kenaf.关键词
红麻/耐盐/抗旱/WD40重复蛋白Key words
Kenaf/Salt tolerance/Drought resistance/WD40 repeat protein分类
农业科技引用本文复制引用
李辉,李德芳,陈安国,唐慧娟,李建军,黄思齐..盐和干旱胁迫下红麻HcWD40-1基因的克隆及表达特征[J].农业生物技术学报,2017,25(12):1970-1978,9.基金项目
国家麻类产业技术体系红麻育种项目(No.CARS-19-E07)和中国农业科学院科技创新工程一年生麻类育种项目(No.ASTIP-IBFC03) （No.CARS-19-E07）
