农业生物技术学报2017，Vol.25Issue(12)：1979-1988,10.DOI:10.3969/j.issn.1674-7968.2017.12.008
从江香猪肌内和皮下脂肪前体细胞分化过程中相关基因的表达
The Expression of Related Genes on Intramuscular and Subcutaneous Preadipocytes During Differentiation in Congjiang Xiang Pig (Sus scrofa)
摘要
Abstract
In order to investigate the differential gene expression on intramuscular and subcutaneous preadipocytes during differentiation in Congjiang Xiang pig (Sus scrofa),the fat precursors culture model in vitro of Congjiang Xiang pig was established.In this study,Congjiang Xiang pig in five days old was provided,then subcutaneous adipose tissue and the longissimus dorsi muscle was excised.We used collagenase to harvest preadipocytes from longissimus dorsi muscle and subcutaneous adipose tissue respectively.The two preadipocytes were induced differentiation by a series of hormone-induced complexes.The mRNA expression levels of gene involved in adipogenesis,such as peroxisome proliferator-activated receptorsγ (PPARγ),lipoprotein lipase (LPL),fatty acid synthase (FAS),acetyl-CoA carboxylase (ACC),adipose triglyceride lipase (ATGL) and Hormone sensitive lipase (HSL),were compared by real-time PCR in the two preadipocytes.The result showed that the model of preadipocytes in vitro was successfully established from Congjiang pig.The mRNA expression levels of PPARy,FAS and ACC in intramuscular preadipocytes were significantly a higher at 72 h after the addition of inducer,which a higher than the level in 0 h (P＜0.05).The mRNA expression level of LPL was no significant difference in the various stages,but there was a significant up-regulation of LPL in 144 h after the addition of inducer,and the value was significantly higher than the other stages (P＜0.01).The mRNA expression levels of ATGL and HSL showed the pattern of "updown-up" in the differentiation phase.In the subcutaneous preadipocytes,the mRNA expression level of PPARγ was the highest in 72 h after induction,and the value was significantly higher than in other stages (P＜0.01).The mRNA expression levels of LPL,FAS,ACC,ATGL and HSL were all higher in 48 and 72 h,and the value was significantly higher than that in 0h (P＜0.01).In summary,this study showed that in intramuscular preadipocytes,the genes related to adipocyte differentiation were more active at the late stage of differentiation,while in subcutaneous preadipocytes,the related genes were more active at the earlier stage of differentiation in vitro.The result prompt that the formation of subcutaneous adipose is much earlier than intramuscular adipose,which may be of great significance in fat deposition mechanism of Congjiang Xiang pig for further study.关键词
从江香猪/肌内脂肪前体细胞/皮下脂肪前体细胞/时序表达Key words
Congjiang Xiang pig/Intramuscular preadipocyte/Subcutaneous preadipocyte/Timing expression分类
农业科技引用本文复制引用
孙成娟,许厚强,赵佳福,陈伟,周迪,张青青..从江香猪肌内和皮下脂肪前体细胞分化过程中相关基因的表达[J].农业生物技术学报,2017,25(12):1979-1988,10.基金项目
国家科技支撑计划(No.2015BAD03B02-3)、贵州畜禽种质资源保存创新与利用(黔科合重大专项字(2013)6008)和贵州大学研究生创新基金(研农2016021) （No.2015BAD03B02-3）
