农业生物技术学报2017，Vol.25Issue(12)：1989-1997,9.DOI:10.3969/j.issn.1674-7968.2017.12.009
三个绵羊品种BMPR-IB基因CDS区1113位点的多态性与产羔数的相关性分析
The Correlation Analysis Between Polymorphism and Fecundity of 1113 Locus in BMPR-IB Gene CDS Area of Three Sheep (Ovis aries)Varieties
摘要
Abstract
The fecundity of sheep (Ovis aries) is controlled by the dominant gene,and the bonemorphogenetic protein type IB gene (BMPR-IB) has been identified as one of the dominant genes in multiparous ewes.To provide a theoretical basis for breeding for sheep fecundity and markers,the polymerase chain reaction single strand conformation polymorphism (PCR) was used to analysis the single nucleotide polymorphism (SNP) detection at 1113 loci in the bonemorphogenetic protein type IB (BMPR-IB) gene coding region of 3 breeds of sheep,including 188 Mongolian sheep,112 Gansu Alpine Merino sheep and 48 Small Tailed Han sheep,then we also analyze the correlation among the polymorphism of 3 breeds of sheep BMPR-IB gene and litter size,in order to provide the material for studying the mechanism of prolificacy in sheep.The result showed that 3 different genotypes were found in Mongolian sheep and Gansu Alpine Merino sheep,including wild type called AA,homozygous mutant called BB and heterozygous mutant called AB,AB was the dominant genotype.2 different genotypes were found in Small Tailed Han sheep,BB and AB,BB was the dominant genotype.With the analysis of polymorphism information content,it was shown that Small Tail Han sheep belonged to low polymorphism (PIC＜0.25),the other two breeds of sheep belonged to moderate polymorphism (0.25＜PIC＜0.5).There was a significant difference in the 1113 locus of BMPR-IB gene coding region among these 3 different breeds of sheep,this site may be related to the litter size of sheep so it can be used as a marker loci for reproductive traits of sheep and further theoretical basis for the production practice.关键词
骨形态发生蛋白受体IB基因(BMPR-IB)/小尾寒羊/蒙古羊/甘肃高山细毛羊/多态性Key words
The bonemorphogenetic protein type IB gene (BMPR-IB)/Small Tail Han sheep/Mongolia sheep/Gansu alpine merino sheep/Polymorphism分类
农业科技引用本文复制引用
杜丽,张利平,张筱艳,朱韶华,马晓明..三个绵羊品种BMPR-IB基因CDS区1113位点的多态性与产羔数的相关性分析[J].农业生物技术学报,2017,25(12):1989-1997,9.基金项目
甘肃农业大学科研项目(No.GSAU-ZL-2015-027)和国家自然科学基金(No.31260547) （No.GSAU-ZL-2015-027）
