水产学报2018，Vol.42Issue(1)：1-9,9.DOI:10.11964/jfc.20161210664
基于线粒体COⅠ的DNA条形码在对虾科种类鉴定中的研究
The research of COⅠ-based DNA barcoding in Penaeidaes' identification
摘要
Abstract
The Penaeidae comprises 26 genera and approximately 200 species as most of Penaeidae shrimps in the same genus are morphologically similar, and just exhibited subtle morphological differences, which makes identi-fication of Penaeidae shrimps a difficult task based on taxonomic keys alone. In order to define the practicability of DNA barcoding in identification of Penaeidae shrimps, in this study, we used COⅠ gene to examine 32 kinds of Penaeidaes' nucleotide composition, interspecific genetic distance and intraspeccific genetic distance, and to build neighbor-joining tree of 32 kinds of Penaeidae based on mtDNA COⅠ gene. As a result, the COⅠ sequences of 32 kinds of Penaeidae present base preference. On average, the content of A+T (61.50%)was significantly higher than that of G+C (38.50%). As calculated by Kimera-2-parameter model, the mean distance within-species was 0.003, the mean distance pairwise-species (0.468)is 156 times greater than the mean distance within-species. The phylogeny showed that 30 kinds of Penaeidae from 32 kinds of Penaeidae could converge upon a monophyly with high support values. We can see the good applicability of mitochondrial COⅠ-based DNA barcoding in Pen-aeidaes' identification and use it as a necessary complement and a test of morphological taxonomic system.关键词
对虾科/DNA条形码/COⅠ基因/鉴别Key words
Penaeidaes/DNA barcoding/COⅠ gene/identification分类
生物科学引用本文复制引用
易啸,王攀攀,王军,苏永全,毛勇..基于线粒体COⅠ的DNA条形码在对虾科种类鉴定中的研究[J].水产学报,2018,42(1):1-9,9.基金项目
国家虾产业技术体系专项(CARS-48) （CARS-48）
福建省科技厅重大专项(2016NZ0001-4) （2016NZ0001-4）
厦门海洋经济创新发展示范项目(16CZY009SF05) （16CZY009SF05）
National Shrimp Industry Technical System Project (CARS-48) （CARS-48）
Major Projects of Science and Technology Department of Fujian Province (2016NZ0001-4) （2016NZ0001-4）
Marine Economy Innovation and Development Demonstration Project of Xiamen (16CZY009SF05) （16CZY009SF05）
