水产学报2018，Vol.42Issue(1)：10-17,8.DOI:10.11964/jfc.20160210286
基于微卫星评估草鱼放流亲本对野生群体遗传多样性的影响
Genetic effect of released brood grass carp (Ctenopharyngodon idella) on wild population in the Yangtze River inferred from microsatellite markers
摘要
Abstract
The natural resources of grass carp (Ctenopharyngodon idella) in Yangtze River have significantly de-creased in the past decades. In recent years, large-scale releases of brood grass carp are carried out for ecological restoration in Yangtze River. In order to assess the genetic effect of released brood fishes on wild population in the middle reaches of Yangtze River, thirteen polymorphism microsatellite markers were selected to genotype the ge-netic differentiation among released and wild populations. Fifteen populations were sampled, including ten re-leased and five wild populations. The results showed that polymorphic information content and gene diversity of the microsatellite loci was 0.8622 (0.657–0.950) and 0.8555 (0.675–0.936), respectively. The number of effective alleles of the 15 populations ranged from 7.4503 to 10.1536, and allelic richness from 11.483 to 15.204, which in-dicated that genetic diversity of grass carp populations was moderately high. The total and pairwise genetic differ-entiation indexes (FST) were low to 5%, loci implying that there was no significant genetic differentiation among populations. The 15 grass carp populations were divided into 4 groups based on Bayesian clustering analysis and principal component analysis. According to above grouping and geographic distribution of the 15 grass carp popu-lations, we have conducted AMOVA analysis, as a result, most of the genetic variation of grass carp populations came from inter-individuals, and the level of genetic differentiation among groups was low (FCT<5%; FSC<5%). In summary, there was hardly any genetic effect of releasing nature grass carp on wild populations in the Yangtze River under current model.关键词
草鱼/微卫星/遗传多样性/增殖放流Key words
Ctenopharyngodon idella/microsatellite/genetic diversity/releasing分类
生物科学引用本文复制引用
季晓芬,段辛斌,刘绍平,陈大庆,汪登强..基于微卫星评估草鱼放流亲本对野生群体遗传多样性的影响[J].水产学报,2018,42(1):10-17,8.基金项目
国家自然科学基金(51579247) （51579247）
National Natural Science Foundation of China (51579247) （51579247）
