水产学报2018，Vol.42Issue(1)：18-28,11.DOI:10.11964/jfc.20170110699
淇河鲫甘露糖受体基因克隆及嗜水气单胞菌感染对其基因表达的影响
Molecular cloning of mannose receptor in Carassius auratus var. Qihe and effects of Aeromonas hydrophila infection on its gene expression
摘要
Abstract
Mannose receptor (MR), a member of the C type lectin receptor superfamily, is thought to be involved in the non-specific immune responses in animals. To illustrate the MR molecular structure of Carassius auratus var. Qihe (CaMR) and the role it played in anti-infection immunity, homology cloning and RACE techniques were used in the present study to obtain the complete cDNA sequence, and the real-time fluorescent quantitative PCR (qPCR) technique was used to measure the effect of Aeromonas hydrophila (A. hydrophila) infection on its gene expression. The full-length cDNA of CaMR contains 4473 bp, including 81bp at 5′UTR and 90 bp at 3′UTR. The open reading frame encodes a putative protein of 1433 aa with a signal peptide of 20 aa. The predicted amino acid sequences showed that CaMR contained a cysteine-rich domain (CRD), a single fibronectin type domain (FNⅡD), eight C-type lectin-like domains (CTLDs), a transmembrane domain and a short C-terminal cytoplasmic domain, sharing highly conserved structures with MRs from the other species. Amino acid sequence homology and phylo-genic tree showed the close relationship between CaMR and other cyprinid fishes. The MR mRNA could be detec-ted in all the examined tissues with highest level in headkidney. The temporal expression patterns of MR in the headkidney, spleen, and heart post of infection with A. hydrophila first rose and then fell, while in the intestine de-creased continually. The current research provides a basis for further revealing the function of CaMR in immune reaction and for its application in disease prevention and treatment of Carassius auratus var. Qihe.关键词
淇河鲫/嗜水气单胞菌/甘露糖受体/基因克隆/基因表达Key words
Carassius auratus var. Qihe/Aeromonas hydrophila/mannose receptor/gene cloning/gene expression分类
生物科学引用本文复制引用
王俊丽,闫潇,卢荣华,秦超彬,梁丽娜,刘燕琴,聂国兴..淇河鲫甘露糖受体基因克隆及嗜水气单胞菌感染对其基因表达的影响[J].水产学报,2018,42(1):18-28,11.基金项目
国家自然科学基金(31372545) （31372545）
河南省高校科技创新团队支持计划(14IRTSTHN013) （14IRTSTHN013）
河南省自然科学基金(162300410165) （162300410165）
河南省科技计划基金项目(142300410159) （142300410159）
河南省教育厅科学技术重点研究项目(14A180007) （14A180007）
National Natural Science Foundation of China (31372545) （31372545）
Program for Innovative Research Team (in Science and Technology) in University of Henan Province (14IRTSTHN013) （in Science and Technology）
Natural Science Founda-tion of Henan Province (162300410165) （162300410165）
Science and Technology Plan Project of Henan Province (142300410159) （142300410159）
Key Scientific and Technological Project of Henan Education Department (14A180007) （14A180007）
