水产学报2018，Vol.42Issue(1)：29-38,10.DOI:10.11964/jfc.20161210644
M、F型COⅡ基因在雌雄三角帆蚌不同发育时期的表达差异研究
Full-length cDNA cloning of M and F-type COⅡ genes and expression in different age freshwater mussel Hyriopsis cumingii
摘要
Abstract
The mitochondrial genetic patterns of bivalve mollusc are different from the traditional maternal inherit-ance. The mitochondria of bivalve molluscs have two kinds of mtDNA of M type and F type, which are called doubly uniparental inheritance (DUI). In order to explore the mechanism of DUI, the full-length cDNA of M and F-type COⅡ genes in Hyriopsis cumingii was obtained by RACE. qRT-PCR was used to detect the expression of M and F-type COⅡ genes in different tissues and stages. The results are as follows: 1.The full-length cDNA se-quences of M-type COⅡ gene and F-type COⅡ gene were 1244 bp and 808 bp respectively, and the 3′ end of M-type COⅡ gene had a prolonged sequence of 546 bp. The transmembrane structure prediction showed that there were four transmembrane structures in the C-terminus extension region of the M-type COⅡ gene, suggesting that this extension sequence has certain function. 2. M-type COⅡ gene and F-type COⅡ gene were all expressed in the early juvenile (embryonic to 5-month-old) of H.cumingii, and the expression level of F-type COⅡ gene was significantly higher than that of M-type COⅡ gene. 3. In the tissues of six-month-old mussels, the expression of M-type COⅡ gene was the highest in gonads, and the expression of F-type COⅡ gene was still at a high level in the same tissues. 4. In the one-year-old female tissues, M and F-type COⅡ genes can be detected in all tissues. In male tissues, the F-type COⅡ gene was detected in each tissues, but M-type COⅡ gene was only expressed in gonads. 5. In the two-year-old female tissues, F and M type both expressed in each tissue, but the expression of M-type COⅡ gene is very low. In the two-year-old male tissues, the F-type COII gene was detectable in addition to the gonad, and the M-type COⅡ gene was detected only in the gonad. In the two-year-old male tissues, the F-type COⅡ gene was detectable except for the gonad, and the M-type COⅡ gene was detected only in the gonad. These results suggested that M-type mtDNA is selectively degraded or aggregated in tissues during male and female de-velopment of H. cumingii.关键词
三角帆蚌/双单亲遗传/COⅡ基因/表达差异Key words
Hyriopsis cumingii/doubly uniparental inheritance (DUI)/COⅡgene/expression differences分类
生物科学引用本文复制引用
陈亚,王雅昱,汪桂玲,何方殊,李家乐..M、F型COⅡ基因在雌雄三角帆蚌不同发育时期的表达差异研究[J].水产学报,2018,42(1):29-38,10.基金项目
国家自然科学基金(31772835) （31772835）
上海市教委科研创新重点项目(13ZZ128) （13ZZ128）
上海高校知识服务平台项目(ZF1206) （ZF1206）
National Natural Science Foundation of China (31101939) （31101939）
Key Fundamental Shanghai Uni-versities Knowledge Service Platform (ZF1206) （ZF1206）
Innovation Program of Shanghai Municipal Education Commis-sion (13ZZ128) （13ZZ128）
