水产学报2018，Vol.42Issue(1)：72-79,8.DOI:10.11964/jfc.20161110601
温度对马来西亚红罗非鱼越冬期体色的影响
Effects of temperature on body color of Malaysian red tilapia during overwintering period
摘要
Abstract
In recent years, red tilapia has been gaining popularity due to its very fast growth, the absence of black peritoneum, salinity tolerance and adaptability to any culture system. However, the pigmentation differentiation in genetic breeding and skin color variation during the overwintering period are the main problems limiting the devel-opment of commercial red tilapia culture. The pigmentation differentiation is not reversible and skin color vari-ation during overwintering period is reversible with the environmental temperature increasing. Coloration patterns including whole pink, pink with scattered black spots and pink with scattered red spots have been found in our breeding population. In order to further understand the genetic molecular mechanism of pigmentation differenti-ation and variation so as to solve the skin color variation during overwintering period, this research compared the effects of different temperatures (16, 20, 25 and 30 °C) on apparent color, tyrosinase (Tyr) activity and skin pig-ment cells of Malaysian red tilapia during overwintering period. After fifty days experiment, we found the body color of most fish in T16 group changed to pewter from pink at the beginning. Most of the abdominal skin color in T20 group fish also varied to pewter. But the fish body color in T25 group had only slight change and the body color of T30 group was still whole pink. The Tyr activity of fish back skin, abdominal skin and serum increased with increasing of temperature, and was the highest in 25 °C group fish. However, the Tyr activity decreased in 30 °C group fish. The blood tyr mRNA expression increased with increasing of temperature, and the muscle tyr mRNA expression in 25 and 30 °C group fish were higher than that in 16 and 20 °C group. The melanocytes of fish dorsal skin decreased in number with increasing of temperature by slice microstructure observation. The res-ults suggest that the color variation during overwintering period of red tilapia may be related to the changes of skin melanocytes and Tyr activity. The further study of the regulatory mechanism would help to understand the genetic mechanism of fish body color and improve the body color features.关键词
红罗非鱼/温度/体色/酪氨酸酶/皮肤显微切片/越冬Key words
red tilapia/temperature/body color/tyrosinase/slice microstructure/overwintering分类
农业科技引用本文复制引用
王兰梅,宋飞彪,朱文彬,董娟娟,傅建军,董在杰..温度对马来西亚红罗非鱼越冬期体色的影响[J].水产学报,2018,42(1):72-79,8.基金项目
江苏省自然科学基金—青年基金(BK20160203) （BK20160203）
中国水产科学研究院基本科研业务费(2017HY-XKQ0203) （2017HY-XKQ0203）
Jiangsu Natural Science Foundation for Young Scholar (BK20160203) （BK20160203）
Central Public-interest Scientific Institution Basal Research Fund, CAFS (2017HY-XKQ0203) （2017HY-XKQ0203）
