水产学报2018，Vol.42Issue(1)：91-99,9.DOI:10.11964/jfc.20170110694
L-色氨酸和褪黑激素对中华绒螯蟹血清血糖水平及肝胰腺抗氧化能力的影响
Effects of L-tryptophan and melatonin on the serum glucose level and antioxidant capacity in the hepatopancreas of Chinese mitten crab (Eriocheir sinensis)
摘要
Abstract
In order to investigate the effects of L-tryptophan and melatonin on the serum glucose level and antiox-idant capacity in the hepatopancreas of Chinese mitten crab (Eriocheir sinensis), the female crabs with the average body weight of (16.58±2.20) g were randomly allocated into 8 groups (the processing experiments of L-trypto-phan and melatonin had 4 groups respectively). Each experimental period was 30d. (1) The feeding experiment of L-tryptophan: crabs were fed with diet which added 4 levels of L-tryptophan, and the L-tryptophan percently for the diet were 0.36% (basal diet, namely control group), 0.47%, 0.73% and 1.05% (named diet 1, 2, 3 and 4, re-spectively). The serum glucose level and T-AOC and the capacity of SOD in the hepatopancreas were determined. (2) The injection experiment of melatonin: on the 1st and 15th days, crabs were injected with saline (control group namely C) and different doses of melatonin which were 10–6 mol/crab (μM), 10–9 mol/crab (nM) and 10–12 mol/crab (pM). Then hemolymph and hepatopancreas were collected separately for the determination of the serum glucose level and T-AOC and the capacity of SOD in the hepatopancreas on the 1st and 15th days. In addition, we analyzed the serum glucose level on the 30th day. The results showed that different levels of L-tryptophan had no significant effect on serum glucose level, and its value was approximately (4.62±0.20) mmol/L. The diet which contained 0.73% L-tryptophan could significantly increase the capacity of SOD (82.86±1.07) U/mL. Injection of melatonin into crabs resulted in hyperglycemia in a dose-dependent manner on the 1st day. Among them, the serum glucose level of μM reached up to (7.56±0.36) mmol/L, which was significantly higher than other three groups. Moreover, the injection of MT played an important role in promoting the T-AOC and the capacity of SOD, but only effective on the 1st day. Compared with C, pM and nM, the the T-AOC and the capacity of SOD of μM was higher than those significantly. In summary, 0.73% L-tryptophan can improve the antioxidant capacity of crabs, but the effects on the serum glucose level are not obvious. The injection of 10–6 mol/crab melatonin can improve the serum gluc-ose level and antioxidant capacity of Chinese mitten crab for short time (1 d).关键词
中华绒螯蟹/L-色氨酸/褪黑激素/血糖水平/抗氧化能力Key words
Eriocheir sinensis/L-tryptophan/melatonin/glucose level/antioxidant capacity分类
农业科技引用本文复制引用
徐敏杰,张佳鑫,黄根勇,张聪,成永旭,杨筱珍..L-色氨酸和褪黑激素对中华绒螯蟹血清血糖水平及肝胰腺抗氧化能力的影响[J].水产学报,2018,42(1):91-99,9.基金项目
国家自然科学基金(31272677,31472287) （31272677,31472287）
上海市科委项目(16DZ2281200) （16DZ2281200）
上海教委知识服务平台专项(ZF1206) （ZF1206）
上海市科技兴农推广项目[沪农科推字(2015)第1-7号] （2015）
National Natural Science Foundation of China (31272677, 31472287) （31272677, 31472287）
Shanghai Municipal Science and Technology Commission (16DZ2281200) （16DZ2281200）
Shanghai Ocean University Aquatic Animal Breeding Cen-ter (ZF1206) （ZF1206）
Key Extension Project (2015-1-7) from Shanghai Agriculture Committee （2015-1-7）
