水产学报2018，Vol.42Issue(1)：100-111,12.DOI:10.11964/jfc.20160810507
氧化鱼油对黄颡鱼幼鱼肠道健康的影响及精氨酸的干预作用
Influence of oxidized fish oil on the intestinal health of juvenile yellow catfish (Pelteobagrus fulvidraco) and the use of arginine as an intervention measure
摘要
Abstract
This experiment was conducted to investigate the influence of dietary oxidized fish oil (OF) and the in-tervention of Arginine (Arg) on the intestinal health of juvenile yellow catfish. A total of 600 healthy yellow cat-fish (Pelteobagrus fulvidraco) with an initial body weight of (4.41±0.05) g were randomly divided into 6 groups with 4 replicates of 25 fish. During 56 d feeding trial, the fish were fed respectively six diets containing 2.5% fresh fish oil (FF), 1.5% FF+1.0% OF (OF1), 0.5% FF+2.0% OF (OF2), 2.5% FF+0.48% Arg (FFA), 1.5% FF+1.0%OF+0.48% Arg (OFA1), 0.5% FF+2.0% OF +0.48% Arg (OFA2). The results showed that the ACP, AKP activit-ies and IL-6 content in intestine of yellow catfish were significantly higher in OF2 group. As for addition of argin-ine to the oxidized fish oil diets, the AKP activity and IL-6 content in intestine of yellow catfish were significantly lower in OFA2 group. The SOD activity in intestine of yellow catfish showed a downward trend and the GSH-PX and CAT activities showed a reversed trend, but there were no significant differences among FF, OF1 and OF2 groups. The SOD activity was significantly higher in OFA2 group. The MDA content in intestine of yellow catfish were significantly higher in OF2 group. Dietary oxidized fish oil and arginine had a significant interaction on the GSH-PX activity in intestine of yellow catfish. The CAT activity in intestine was significantly decreased by the ar-ginine. The DAO activity in intestine of yellow catfish showed a downward trend and the iNOS showed an up-ward trend, but there was no significant difference among FF, OF1 and OF2 groups. The DAO activity signific-antly increased and iNOS significantly decreased in OFA2 group. The arginine had a significant impact on the DAO and iNOS activities in intestine of yellow catfish. The height of folds, the thickness of muscles and the num-ber of goblet cells in foregut increased in OF1 group. In comparison with OF2 group, the height of folds and the number of goblet cells in foregut increased in the OFA2 group. Oxidized fish oil showed a significant influence on the height of folds. The results suggest that dietary oxidized fish oil would reduce the intestinal immune and total antioxidant capacity, and damage the structure in foregut of juvenile yellow catfish, but arginine supplementation can alleviate the inhibition induced by oxidized fish oil for intestinal immune, antioxidant capacity and tissue struc-ture.关键词
黄颡鱼/氧化鱼油/精氨酸/肠道/免疫指标/抗氧化指标Key words
Pelteobagrus fulvidraco/oxidized fish oil/arginine/intestine/immune/antioxidant分类
农业科技引用本文复制引用
卓丽欣,赵红霞,黄燕华,曹俊明,王国霞,陈冰,孙育平..氧化鱼油对黄颡鱼幼鱼肠道健康的影响及精氨酸的干预作用[J].水产学报,2018,42(1):100-111,12.基金项目
国家自然科学基金(31402307) （31402307）
National Natural Science Foundation of China (31402307) （31402307）
