水产学报2018，Vol.42Issue(1)：112-119,8.DOI:10.11964/jfc.20161210655
一种佐剂新材料在嗜水气单胞菌灭活疫苗浸泡免疫 异育银鲫中的应用效果
Efficacy of inactivated Aeromonas hydrophila vaccine with a new biodegradable material adjuvant in Carassius auratus gibelio by immersion immunization
摘要
Abstract
Castor oil polyethylene glycol monoester glucoside (CPMG) is a kind of biodegradable and artificial material. Formalin-inactivated Aeromonas hydrophila bacteria with or without CPMG, CPMG + anisodamine mix-ture were administered on days 1 and 7 to Carassius auratus gibelio through immersion. Spleens were sampled on days 2, 4, 7, 11, 14 and 21 post-second vaccination, and the expression quantities of IgM, IL-1β, C3, C-lectin and lysozyme mRNAs in spleen were detected through the real-time fluorescence quantitative PCR (RT-PCR). Formal-in-inactivated A. hydrophila vaccine with CPMG + anisodamine mixture was immersion administered just once, then, specific antibodies were detected and the fish were challenged intraperitoneally with lethal A. hydrophila at different time post-vaccination. The results of vaccination twice showed that fish immunized with inactivated A. hydrophila vaccine and CPMG exhibited enhanced expression of genes that are involved in both innate and ad-aptive immune responses (IgM, IL-1β, C3, C-lectin and lysozyme) compared to no adjuvant control or saline con-trol. The relative percent survival (RPS) was found to be 70.6% on day 30 post-vaccination, while immunization with the antigen alone followed by an experimental challenge gave an RPS of 56.0%, moreover, the CPMG + an-isodamine coupled vaccine showed an RPS of 88.2%.The results of vaccination just once showed that the CPMG +anisodamine coupled vaccine could augment the production of specific serum antibodies, which reached the highest level 6 weeks post-vaccination. The resulting RPS calculated was 54.6%, 44.0% and 12.5% on days 30, 60, 120 post-vaccination, respectively. These results indicate that formalin-inactivated A. hydrophila vaccine with CP-MG immersion administered to gibel carps can significantly promote the gene expression of IgM, IL-1β, C3, C-lectin and lysozyme in spleen and enhance specific immune responses against A. hydrophila infection.关键词
异育银鲫/蓖麻油聚乙二醇单酯葡萄糖苷/山莨菪碱/灭活疫苗/浸泡免疫Key words
Carassius auratus gibelio/castor oil polyethylene glycol monoester glucoside (CPMG)/anisodamine/formalin-inactivated vaccine/immersion immunization分类
农业科技引用本文复制引用
任燕,时云朵,石存斌,吴淑勤..一种佐剂新材料在嗜水气单胞菌灭活疫苗浸泡免疫 异育银鲫中的应用效果[J].水产学报,2018,42(1):112-119,8.基金项目
广东省海洋渔业科技与产业发展专项(A201501C12) （A201501C12）
现代农业产业技术体系建设专项资金(CARS-46) （CARS-46）
国家科技支撑计划(2012BAD25B02) （2012BAD25B02）
Special Project of Marine Fishery Technology and Industrial Development of Guangdong Province (A201501C12) （A201501C12）
Earmarked Fund for China Agriculture Research System (CARS-46) （CARS-46）
National key Tech-nologies R & D Program of China (2012BAD25B02) （2012BAD25B02）
