水产学报2018，Vol.42Issue(1)：120-130,11.DOI:10.11964/jfc.20161110616
中华鲟脑膜败血伊丽莎白菌的分离鉴定及药敏特性
Isolation, identification and antibiotic sensitivity of Elizabethkingia meningoseptica from Chinese sturgeon (Acipenser sinensis)
摘要
Abstract
To define the potential pathogens from a dead F2 Chinese sturgeon (Acipenser sinensis), which was reared in Taihu hatchery, a Chinese sturgeon breeding site located in Jingzhou City of Hubei Province, China, a bacterial strain EM was isolated from the liver, spleen and ascites of moribund F2 Chinese sturgeon by traditional method of pathogen isolation. The strain EM was identified as Elizabethkingia meningoseptica by means of the 16S rDNA sequence, constructing phylogenetic tree and Biolog Microbial Identification System. The pathogeni-city of the strain EM was confirmed by the infection experiment in healthy F2 Dabry's sturgeon (Acipenser dabry-anus). Meanwhile, histopathological analysis was performed in the sick F2 Chinese sturgeon, and the antibiotic susceptibility test of the strain EM was carried out to guide the clinical medication. The results found the hemosid-erin deposition, ballooning degeneration and hemolysis severely in liver, spleen and kidney of the sick F2 Chinese sturgeon. The strain EM was confirmed to be pathogenic to healthy Dabry's sturgeon by infection experiment, and the same bacterium could be recovered from these infected Dabry's sturgeon. The susceptibility test to antibiotics demonstrated that the strain EM from sick Chinese sturgeon was susceptible to minocycline, clindamycin, clarith-romycin and midecamycin; medium susceptible to tetracycline, cefotaxime and cefoxitin, but resistant to other 28 kinds of antibiotics, which implied EM strain was resistant to multiple antibiotics. In short, the study reported for the first time that Chinese sturgeon could be infected by Elizabethkingia meningoseptica, which was also patho-genic to Dabry's sturgeon. Preliminary study on biological characteristics, antibiotic susceptibility of Elizabeth-kingia meningoseptica, histopathological features of diseased F2 Chinese sturgeon, may provide a theory basis for the diagnosis, prevention and treatment of this disease.关键词
中华鲟/脑膜败血伊丽莎白菌/分离鉴定/药敏试验/病理特征Key words
Acipenser sinensis/Elizabethkingia meningoseptica/isolation and identification/antibiotic susceptib-ility test/histopathological features分类
农业科技引用本文复制引用
邸军,张书环,黄君,杜浩,周勇,危起伟..中华鲟脑膜败血伊丽莎白菌的分离鉴定及药敏特性[J].水产学报,2018,42(1):120-130,11.基金项目
国家"九七三"重点基础研究计划(2015CB150702) （2015CB150702）
National Basic Research Project of China (973 Program) (2015CB150702) （973 Program）
