水产学报2018，Vol.42Issue(1)：131-140,10.DOI:10.11964/jfc.20170510843
湖北省潜江地区克氏原螯虾白斑综合征PCR诊断及 组织病理学观察
Histopathological study and PCR detection of white spot syndrome virus from infected Procambarus clarkii
摘要
Abstract
In May 2016, a severe infectious disease occurred among the farmed Procambarus clarkii in Qianjiang, Hubei Province. Histological examination, virus isolation, nested-PCR. artificial infection experiment and phylo-genetic analysis were performed to explore its causes and the epidemic law. Principal symptoms included darken-ing of the body, reduction in vigour, low feeding. Histopathologically, hepatopancreas, intestines, muscle, gill showed varying degrees of degeneration, necrosis and inflammatory cellinvasion. Pathologic changes were the same as the white spot syndrome virus (WSSV) infected arthropod. PCR detection results also showed that tissues of dying P. clarkii were positive to WSSV, the positive rates of PCR was 55.56%(15/27), while those were negat-ive to monodon baculovirus disease (MBV) and infectious hypodermal and hematopoietic necrosis (IHHNV). P. clarkii that died in oral infection was positive for the presence of WSSV by Nested-PCR.The sequenced ampli-fication products shared 100% identity with white spot syndrome virus isolate EG3, complete genome (GenBank access number: KR083866.1). Phylogenetic analysis showed that the isolate was belonging to WSSV. The hepato-pancreas, intestine and muscle tissue of the diseased P. clarkii were fed to the healthy P. clarkii. The results showed that a cumulative mortality of 100% was observed in the infected P. clarkii, while no death in control group. Therefore WSSV was determined to be the pathogen of the P. clarkii.关键词
克氏原螯虾/白斑综合征/组织病理学Key words
Procambarus clarkia/white spot syndrome virus/histopathological分类
农业科技引用本文复制引用
何琦瑶,汪开毓,刘韬,刘绍春,贺扬,叶彩燕,何晟毓,秦振阳,谢恒..湖北省潜江地区克氏原螯虾白斑综合征PCR诊断及 组织病理学观察[J].水产学报,2018,42(1):131-140,10.基金项目
四川省科技厅应用基础项目(2014JY0143) （2014JY0143）
教育部"长江学者和创新团队发展计划"创新团队项目(IRT0848) （IRT0848）
Sichuan Science and Technology Agency Application Foundation Project (2014JY0143) （2014JY0143）
Pro-gram for Changjiang Scholars and Innovative Research Teams in the University (IRT0848) （IRT0848）
