生物医学工程研究2017，Vol.36Issue(4)：356-360,5.DOI:10.19529/j.cnki.1672-6278.2017.04.16
以脑源性神经营养因子等干预视神经损伤大鼠视神经拉伸力学的特性分析
The Stress Relaxation and Creep Analysis of Optic Nerve lnjuried Rats Treated with Brain Derived Neurotrophic Factor
摘要
Abstract
To study and judge the intervening effect of nerve injury animal model by comparing the tensile mechanical properties of human umloilical cord blood stem cells and brain derived neurotrophic factor .The optic nerve injury model in rats was prepared by for-ceps operation and was treated respectively by human umbilical cord blood stem cells and brain derived neurotrophic factor after estab -lishing the model 7d.We took out the intraorbital segment of optic nerve after establishing the model 30 d,and histological observation and tensile mechanical properties were experimented .The resuts showed that the animal model of optic nerve injury in BDNF group form was helpful to the recovery of optic nerve axons ,nerve fibers were arranged in order ,a small amount of glial cell vacuolar nucleus ,irreg-ular arrangement,but the diameter of optic nerveaxons did not change significantly .In the animal model of optic nerve injury ,the ar-rangement of optic nerve fibers in the hUCBSC intervention group was more dense ,and most of the axons were normal .All animal exper-imental results showed that ,the maximum load,the maximum stress,elastic limit load and elastic limit stress of the optic nerve injury grou was less than that of the optic nerve injury .model treated with hUCBSC and BDNF,the difference was significant(P<0.05).The maximum load,the maximuns stress,the maximun strain,the elestic limit load and elastic limit stress of optic nerve injury model treated with BDNF was less than that of the one treated with hUCBSC ,the difference was significant (P<0.05).The tensile mechanical proper-ties was improved significantly of the optic nerve injury model treated with BDNF and hUCBSC .It has significant effect that the optic nerve injury rats treated with BDNF ,hUCBSC.关键词
大鼠/视神经损伤/脑源性神经营养因子/人脐血干细胞/组织形态观察/拉伸/力学性能/对比分析Key words
Rats/Optic nerve injury/Brain derived neurotrophic factor/Human umbilical cord blood stem cells/Tissue morphology observation/Tensile/Mechanical properties/Comparative analysis分类
医药卫生引用本文复制引用
王玲,吕雪漫,姜琦..以脑源性神经营养因子等干预视神经损伤大鼠视神经拉伸力学的特性分析[J].生物医学工程研究,2017,36(4):356-360,5.基金项目
吉林省科技发展计划资助项目(20110492). （20110492）
