上海海洋大学学报2017，Vol.26Issue(6)：862-871,10.DOI:10.12024/jsou.20170302001
混养池塘生物膜微生物群落功能多样性特征分析
Microbial metabolic characteristics of biofilm communities in polyculture pond
摘要
Abstract
The microbe attached in biofilm carrier material could ulitize different carbon sources in high yield collection pond.In order to understand the metabolic characteristics of these microbes,the Biolog-ECO technology and water quality analysis were used to analyze the change of microbial carbon metabolism and water quality during the formation of biofilm (0-9 w) in high collection pond including grass carp and tilapia.The results showed that:(1) During the test,using biofilm in polyculture ponds has good removal effect of total nitrogen and phosphorus,and has good effects on maintaining water quality stability;(2) For different sampling time points,the metabolic activity of ulitizing carbon source displayed obvious difference:3,5,7,8 w biofilm metabolic activity at high levels;In the biofilm formation process,the trend of the biofilm microbial metabolic activity first rose and then reduced;Richness index,Shannon index,Simpson index,and McIntosh index performance for 3,4,5,7,8 w were significantly higher than other weeks;(3)Studies on the use of six kinds of carbon sources indicate that biofilm microbes have the highest utilization rates for carbohydrates and polymers;Along with the formation of the biofilm,adherent microbes of glycogen increased obviously,such as n-acetyl-D-glucosamine,beta-methyl-D-glucoside,1-phosphate glucose utilization of carbon sources;(4) Biofilm microbial metabolic characteristics of principal component analysis (PCA) shows that the different sampling time points of samples gathered in three different areas,including 3,4,6,8,9 w microbial metabolic difference is small,0w and l w as a whole,5 w and 7 w as a whole,but there were significant differences between the three concentrated areas.The resultswould provide some reference for further utilization of carbon sources to strengthen biofilm formation in aquaculture.关键词
高产混养池塘/生物膜/Biolog-ECO/碳代谢特征Key words
high-yield polyculture pond/biofilm/Biolog-ECO/carbon metabolism分类
农业科技引用本文复制引用
车建锋,李志斐,王广军,陈成勋,谢骏,郁二蒙,张凯..混养池塘生物膜微生物群落功能多样性特征分析[J].上海海洋大学学报,2017,26(6):862-871,10.基金项目
广东省自然科学基金(2015A030313699) （2015A030313699）
广东省省级科技计划项目(2016A020210023) （2016A020210023）
中国水产科学研究院基本科研业务费专项(2016HY-JC02-04) （2016HY-JC02-04）
国家大宗淡水鱼产业技术体系(CARS-45-21) （CARS-45-21）
