世界中医药2017，Vol.12Issue(12)：2889-2891,2896,4.DOI:10.3969/j.issn.1673-7202.2017.12.006
知柏地黄丸对阴虚"上火"证血清抗炎凝血蛋白的影响
Effect of Zhibai Dihuang Granule on Anti-inflammatory and Clotting Serum Proteins in Yin-deficiency Shanghuo Syndrome
摘要
Abstract
Objective:To explore the influence of Zhibai Dihuang Granule ( ZDG) on the anti-inflammatory clotting serum proteins in Yin-deficiency Shanghuo ( YDS) syndrome.Yin-deficiency SD rats were induced by intraperitoneal injection of Triiodothyronine (T3).The models of gingivitis in rats were induced by dropwise add of porphyromonas gingivalis model.YDS syndrome rats were treated with ZDG for 1 week.iTRAQ-2DLC-MS/MS was used in screening and identifying the serum differential proteins.With the treatment of ZDG,the serum level of Gelsolin (Gsn),Plasminogen (Plg),Fibnnogenalpha chain (Fga),and Thymus beta-4 (T-β4) were significantly increased (fold change>1.25),while the serum level of Collagulation factor XIII Achain and Colla 2 were significantly decreased (fold change<0.8).Gsn possesses the function of removing damaged tissue,releasing the actin,inhibi-ting the apoptosis of the cells,and inhibiting the apoptosis of the cells.Colla2 and Plgcan regulated the change of blood rheology in the inflammatory site.Gsn and T-beta 4 presented the ability in regulating inflammatory cytokines to promote the healing of in-flammatory damage.Conclusion:ZDG may play an important role in clearing actin and lipopolysaccharide released by damage tis-sue and inhibiting the cell apoptosis.Besides,ZDG may regulate inflammatory factors,change the blood rheology of the inflamma-tory site,promot the healing of inflammatory damage.In short,ZDG treats YDH syndrome by regulating these proteins associated with anti-inflammatory and clotting.关键词
知柏地黄丸/阴虚"上火"/蛋白组学/抗炎/凝血/发病机制Key words
Zhibai Dihuang Granule/Yin-deficiency Shanghuo syndrome/Proteomics/Anti-inflammatory/Clotting/Pathogenesis分类
医药卫生引用本文复制引用
毛连根,李仲杰,李继承,刘昌铭,杨粟,江婷婷,陈钟梁,屠慧惠,陈静,胡玉婷,甘霖..知柏地黄丸对阴虚"上火"证血清抗炎凝血蛋白的影响[J].世界中医药,2017,12(12):2889-2891,2896,4.基金项目
国家重点基础研究发展计划(973计划)项目(2014CB543002) （973计划）
