世界中医药2017，Vol.12Issue(12)：3050-3054,5.DOI:10.3969/j.issn.1673-7202.2017.12.048
黄芪注射液联合葛根素注射液对2型糖尿病动物KKA y小鼠心脏组织IRE1α和XBP1表达的影响
Effects of Huangqi Injection Combined with Puerarin Injection on KKAy Mice with Diabetic Cardiomyopathy on Endoplasmic Reticulum Stress
摘要
Abstract
Objective:To investigate the cardioprotective mechanism of Huangqi injection combined with puerarin injection on KKAy mice with diabetic cardiomyopathy,providing experimental evidence for the treatment of diabetic cardiomyopathy using tradi -tional Chinese medicine.Methods KKAy mice were randomly divided into model group and treatment group ( Huangqi injection combined with puerarin injection ) according to blood glucose.The male C57BL/6J mice with same age were used as normal con-trol group.The mice were sacrificed at 20,24 and 28 weeks old,respectively.qRT-PCR was used for detecting the mRNA expres-sion of IRE1α,XBP1U and XBP1S and Western blotting was used for assaying the expression of IRE 1αand XBP1.Results:The mRNA and protein expression of IRE1αin the model group increased at 20,24 and 28 weeks,while the increase of mRNA and protein expression of IRE1αreduced in the treatment group at 20,24 and 28 weeks.The difference was statistically significant (P<0.05).The mRNA and protein expression of XBP1S at 20,24 and 28 weeks and the mRNA expression of XBP 1U at 28 weeks was increased in the model group;For the treatment group,the mRNA expression of XBP1S at 20 and 40 weeks and protein ex-pression of XBP1 was down-regulated,whereas the expression of XBP1S and XBP1U mRNA increased.The difference was statisti-cally significant ( P <0.05 ).Conclusion:Huangqi injection combined with puerarin injection could inhibit the expression of IRE1αand XBP1,two stress signaling molecules of endoplasmic reticulum in the heart tissue of KKAy mice,and reduce the endo-plasmic reticulum stress and exert cardioprotective effect on KKAy mice with type 2 diabetes.关键词
黄芪注射液/葛根素注射液/糖尿病心肌病/内质网应激Key words
Huangqi injection/Puerarin injection/Diabetic cardiomyopathy/Endoplasmic reticulum stress分类
医药卫生引用本文复制引用
张淑静,王谦,李姝玉,赵福建,王岩飞,高誉珊,李奕萱,张达..黄芪注射液联合葛根素注射液对2型糖尿病动物KKA y小鼠心脏组织IRE1α和XBP1表达的影响[J].世界中医药,2017,12(12):3050-3054,5.基金项目
北京市高校教师青年英才计划项目(YETP0794) （YETP0794）
