中国生态农业学报2018，Vol.26Issue(1)：8-15,8.DOI:10.13930/j.cnki.cjea.170794
转基因水稻秸秆还田对土壤硝化反硝化微生物群落的影响
Effect of transgenic rice straw return to soil on nitrification and denitrification microbial community
摘要
Abstract
The cultivation of genetically modified plants may have the potential to alter microbial community structure and function in soils through root exudates and plant residues. Ammonia-oxidizing bacteria or denitrifying bacteria are the key microbes for nitrification or denitrification. A change in community structure of ammonia-oxidizing bacteria or denitrifying bacteria can affect the conversion and utilization of nitrogen in soil. The purpose of this study was to explore the possibility of transgenic rice to induce change in nitrogen transformation and N2O emission in paddy soils. In the study, the abundance and composition of ammonia-oxidizing bacteria or denitrifying bacteria in paddy soils under straw return to soil undercry1Ac/cpti transgenic gene rice 'Kf8' or non-transgenic rice 'Mh86' were analyzed by real-time PCR and denaturing gradient gel electrophoresis (DGGE) based on amoA gene or nirS gene. The results showed that there were no differences in the abundance of amoA gene or nirS gene in the soil with returned transgenic rice 'Kf8' straw and non-transgenic rice 'Mh86' straw. The abundance of amoA gene in the 0–10 cm soil layer was significantly (P< 0.05) higher than that in the 10–20 cm and 20–30 cm soil layers under degraded transgenic rice 'Kf8' straw or non-transgenic rice 'Mh86' straw. The abundance of nirS gene in the soil increased with the time of returned straw to soil for either transgenic rice 'Kf8' or no-transgenic rice 'Mh86'. At the same time, the Shannon-Weiner index and composition of ammonia-oxidizing bacteria or denitrifying bacteria in the soil under degraded transgenic rice 'Kf8' straw were similar to those under degraded non-transgenic rice 'Mh86' straw. The composition of ammonia-oxidizing bacteria in the soil was significantly correlated with the time of rice straw return (P= 0.002). Also the abundance of denitrifying bacteria was significantly correlated with the time of rice straw return (P= 0.002) and depth of soil (P= 0.024). The findings demonstrated that there was no significant effect of returnedcry1Ac/cpti transgenic rice straw to soil on key microbial communities for nitrification or denitrification in soil. In terms of soil microbial community, there was no significant effect of returned cry1Ac/cpti transgenic rice straw to soil on nitrogen transformation and N2O emission in paddy soils.关键词
转基因水稻/秸秆还田/氨氧化细菌/amoA基因/反硝化细菌/nirS基因/群落结构Key words
Transgenic rice/Straw return/Ammonia-oxidizing bacteria/amoA gene/Denitrifying bacteria/nirS gene/Com-munity structure分类
农业科技引用本文复制引用
王沛譞,徐焱,宋亚娜..转基因水稻秸秆还田对土壤硝化反硝化微生物群落的影响[J].中国生态农业学报,2018,26(1):8-15,8.基金项目
福建省自然科学基金项目(2015J01105)、福建省公益类科研院所专项(2015R1019-12)和第30届福建省青少年创新大赛项目资助 This study was supported by the Natural Science Foundation of Fujian Province (2015J01105), the Public Welfare Foundation of Fujian Province (2015R1019-12) and the 30th Youth Innovation Competition Project in Fujian Province. （2015J01105）
