实用医学杂志2017，Vol.33Issue(23)：3858-3863,6.DOI:10.3969/j.issn.1006-5725.2017.23.003
竹节香附素A对肠癌HT29细胞凋亡及周期的影响
Effects on apoptosis and cell cycle of RA in HT29 cells
摘要
Abstract
Objective To investigate the effects of Raddeanin A(RA)on apoptosis and cell cycle of hu-man colon cancer HT29 cells,and discuss the possible mechanism.Methods Different concentration of RA 1,2, 4,8,16μmol/L disposed HT29 cells 12,24,48 hour,3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bro-mide(MTT)assay was used for the cell proliferation;Hoechst33258 staining was performed to observe the effects of RA on apoptosis morphology;Flow cytometry(FCM)was devoted to calculate apoptosis rate and detect cell cycle;The expression of Cleaved-caspase-3,Cleaved-PARP,CyclinB,CDK1 proteins and other proteins were tested by Western blot(WB). Results RA could inhibit the proliferation of HT29 cells,and associated with concentration and time(P<0.05);The IC50of 12 h,24 h,48 h were(9.31 ± 0.63)μmol/L,(4.88 ± 1.02)mol/L,(3.60 ± 0.89) μmol/L.The nucleus pycnosis,fracture were detected by Hochest33258 staining,RA induced apoptosis in HT29 cells;Western blot(WB)assay showed that the expression of Cleaved-caspase-3,Cleaved-PARP rised.HT29 cells were arrested at the period of G2/M,and the CyclinB,CDK1 proteins down-regulated.WB result showed that PI3K, AKT proteins up-regulated,while p-PI3K,p-AKT protein down-regulated.Conclusion RA can inhibit the prolifer-ation of colon cancer HT29 cells and its mechanism is probably by regulating the PI3K/AKT signaling pathways which through inducing apoptosis and blocking the cell cycle.关键词
竹节香附素A/HT29细胞/细胞凋亡/细胞周期/PI3K/AKT信号通路Key words
RA/HT29 cells/apoptosis/cell cycle/PI3K/AKT signaling pathway引用本文复制引用
孟春芹,王瑞平..竹节香附素A对肠癌HT29细胞凋亡及周期的影响[J].实用医学杂志,2017,33(23):3858-3863,6.基金项目
国家中医临床研究基地业务建设科研专项课题(编号:JDZX2012087) （编号:JDZX2012087）
江苏省中医药领军人才项目( 编号:LJ200908) （ 编号:LJ200908）
江苏省卫生计生委课题(编号:BJ14013) （编号:BJ14013）
江苏省普通高校研究生科研创新计划项目(编号:KYLX16-1145) （编号:KYLX16-1145）
