实用医学杂志2017，Vol.33Issue(23)：3867-3870,4.DOI:10.3969/j.issn.1006-5725.2017.23.005
雷公藤甲素对乳腺癌MCF-7细胞增殖和凋亡的影响及其机制
Effects and the mechanism of triptolide on proliferation and apoptosis of breast cancer MCF-7 cells
摘要
Abstract
Objective To explore the effects and mechanism of triptolide on proliferation and apoptosis of breast cancer MCF-7 cells.Methods MCF-7 cells were treated by different concentrations of triptolide.CCK-8 as-say was employed to detect the cell proliferation. The morphological changes were observed by an inverted micro-scope.The apoptosis rate was detected by flow cytometry.Expressions of Bcl-2,Bax,Survivin and Caspase-3 were measured by qRT-PCR and Western blot. Results Triptolide inhibited the proliferation of MCF-7 cells in a dose and time-dependent manner at a suitable range.Triptolide induced morphological changes and apoptosis.Triptolide also down-regulated Bcl-2 and Survivin expressions and up-regulated Bax and Caspase-3 expressions. Conclu-sions Triptolide inhibits proliferation and induces apoptosis of MCF-7 cells,and its mechanism may be related to down-regulation of Bcl-2 and Survivin expressions and up-regulation of Bax and Caspase-3 expressions.关键词
雷公藤甲素/乳腺癌/凋亡/Bcl-2/Bax/Survivin/Caspase-3Key words
Triptolide/Breast cancer/Apoptosis/Bcl-2/Bax/Survivin/Caspase-3引用本文复制引用
陈茂剑,肖潺潺,王丽,杨伟萍,覃庆洪,韦长元..雷公藤甲素对乳腺癌MCF-7细胞增殖和凋亡的影响及其机制[J].实用医学杂志,2017,33(23):3867-3870,4.基金项目
国家自然科学基金资助项目基金资助项目(编号:81360396) （编号:81360396）
2017广西研究生教育创新计划项目基金资助项目(编号:YCSW2017108) （编号:YCSW2017108）
