实用医学杂志2017，Vol.33Issue(24)：4048-4052,5.DOI:10.3969/j.issn.1006-5725.2017.24.007
自噬抑制联合抗-VEGF对视网膜血管内皮细胞血管生成的作用
Role of combination of autophagy inhibition and anti-VEGF in angiogenesis of retinal vascular endothelial cells
摘要
Abstract
Objective In this study,we explored the role of combination of autophagy inhibition and anti-VEGF in proliferation,migration and tube formation of mouse retinal vascular endothelial cells(RVECs). Methods Well cultured mouse RVECs were randomly divided into four groups:autophagy inhibition group(add-ing autophagy inhibitor 3-MA),anti-VEGF group(adding anti-VEGF-A neutralized antibody),autophagy inhibi-tion+anti-VEGF group(adding the two reagents)and the control group.All cells were then cultured in the hypoxic condition. The cell proliferation,migration and tube formation were detected by EdU,transwell and matrigel as-say,respectively. Results The cell proliferation rate,number of migrated cells and number of tube formation of the other three groups decreased when compared with the control group.These data above in autophagy inhibition+anti-VEGF group were all significantly less than 3-MA group and anti-VEGF group. Conclusion Combination of autophagy inhibition and anti-VEGF may be more effective than simple anti-VEGF in inhibition of retinal neovascu-larization.关键词
血管生成/视网膜新生血管/自噬/VEGF/治疗Key words
angiogenesis/retinal neovascularization/autophagy/VEGF/treatment引用本文复制引用
陈萌,李蓉,姚杨,蔡天志,于照祥,林小波..自噬抑制联合抗-VEGF对视网膜血管内皮细胞血管生成的作用[J].实用医学杂志,2017,33(24):4048-4052,5.基金项目
国家自然科学基金项目(编号:81500726) （编号:81500726）
