实用医学杂志2017，Vol.33Issue(24)：4105-4109,5.DOI:10.3969/j.issn.1006-5725.2017.24.021
DNMT1和CCNA1在不同级别宫颈病变组织中的表达及临床意义
Expression and clinical significance of DNMT1 and CCNA1 in different grades of cervical lesions
摘要
Abstract
Objective To investigate the expression and clinical significance of DNMT1 and CCNA1 in different grades of cervical lesions. Methods Cervical tissues were selected from normal cervical(NC),low-grade cervical intraepithelial lesion(LSIL),high-grade cervical intraepithelial lesion(HSIL)and cervical squa-mous cell carcinoma(SCC)from each thirty patients.The expression of DNMT1 and CCNA1 mRNA and protein was examined by Western Blot analysis and qRT-PCR in cervical tissues. Results The expression of DNMT1 mRNA and protein in LSIL,HSIL and SCC was higher than in NC(F=117.93,P<0.05;F=61.24,P<0.05). Expression of DNMT1 mRNA and protein was increased steadily according to severity of cervical lesions(χ2trend=26.25,P<0.05;χ2trend=26.60,P<0.05).The expression of CCNA1 mRNA and protein in HSIL and SCC was lower than that in NC and LSIL(F = 77.04,P < 0.05;F = 57.15,P < 0.05). Expression of CCNA1 mRNA and protein was decreased steadily according to severity of cervical lesions(χ2trend=64.19,P<0.05;χ2trend=60.24,P<0.05). There was a negative correlation between expression of DNMT1 and CCNA1 protein in LSIL,HSIL,SCC (r=-0.75,-0.56,P<0.05). Conclusion In DNMT1 mRNA there is high expression of protein and in CCNA1 mRNA there is low expression of protein,but both may be related to the occurrence and development of cervical cancer.关键词
DNA甲基转移酶1/细胞周期蛋白A1/宫颈癌/宫颈癌前病变Key words
DNA methyltransferase 1/Cyclin A1/cervical cancer/cervical precancerous lesions引用本文复制引用
曹佳宇,白洁,董建新,邵婷,陈燕..DNMT1和CCNA1在不同级别宫颈病变组织中的表达及临床意义[J].实用医学杂志,2017,33(24):4105-4109,5.基金项目
河北省医学科学研究重点课题计划项目(编号:20160726) （编号:20160726）
