西安交通大学学报（医学版）2018，Vol.39Issue(1)：26-30,5.DOI:10.7652/jdyxb201801007
一氧化氮对自发性高血压大鼠外周血T淋巴细胞亚群的影响
Effects of NO on T lymphocyte subsets in peripheral blood of spontaneously hypertensive rats
摘要
Abstract
Objective To investigate whether NO can relieve hypertensive cerebrovascular and renal injury by regulating the ratio of peripheral blood T lymphocyte subsets and reducing proinflammatory cytokine release . Methods Twelve-week SHR and WKY rats were randomly divided into three groups :WKY group ,SHR group and SHR+NO intervention group ,with 6 rats in each group .Rats in SHR+ NO intervention group were injected intraperitoneally with sodium nitroprusside according to 10 μg/(kg · d) for 4 weeks ,while WKY group and SHR group were injected intraperitoneally with an equal amount of saline for 4 weeks .After measuring the tail artery blood pressure ,basilar artery and renal pathological changes were observed by HE staining ;the rates of CD4+ /CD8+ and CD4+ CD25+ T cells were detected by flow cytometry ;and the expression of TNF-α was detected by ELISA .Results The expressions of CD4+ /CD8+ and TNF-αin SHR group were significantly higher than those in WKY group (P<0 .01) while the rate of CD4+ CD25+ in SHR group was significantly lower than that in WKY group (P<0 .01) .The expressions of CD4+ /CD8+ and TNF-α in SHR+ NO intervention group were significantly lower than those in SHR group whereas the rate of CD4+ CD25+ was significantly higher than that in SHR group (P<0 .05) .Conclusion NO can improve the target organ damage caused by hypertension by regulating the peripheral blood T lymphocyte subsets ratio and reducing the release of proinflammatory factors .关键词
自发性高血压大鼠/T淋巴细胞/一氧化氮(NO)/肿瘤坏死因子-α(TNF-α)Key words
spontaneously hypertensive rat/T lymphocyte/NO/TNF-α分类
医药卫生引用本文复制引用
申土旺,单莉娅,于秀石,彭珉,倪欣,李丽,司军强,李新芝,马克涛..一氧化氮对自发性高血压大鼠外周血T淋巴细胞亚群的影响[J].西安交通大学学报（医学版）,2018,39(1):26-30,5.基金项目
国家自然科学基金资助项目(No.81660271) （No.81660271）
石河子大学国际合作项目(No.GJHZ201603) （No.GJHZ201603）
石河子大学应用基础研究青年项目(No.2015ZRKXYQ-LH13 ,2015ZRKXYQ-LH12) （No.2015ZRKXYQ-LH13 ,2015ZRKXYQ-LH12）
兵团中青年科技创新领军人才计划(No.2016BC006)Supported by the National Natural Science Foundation of China (No.81660271 ) , International Cooperation Project of Shihezi University (No.GJHZ201603) ,the Basic Research on Application of Shihezi University Youth Project (No.2015ZRKXYQ-LH13 , 2015ZRKXYQ-LH12) ,and Corps Young and Middle-aged Scientific and Technological Innovation Leading Talent Program (No. 2016BC006) （No.2016BC006）
