中国组织工程研究2017，Vol.21Issue(34)：5423-5429,7.DOI:10.3969/j.issn.2095-4344.2017.34.003
单一生物材料聚乳酸/羟基乙酸共聚物构建组织工程骨软骨的可行性
Feasibility of constructing a scaffold for osteochondral tissue engineering using poly(lactide-co-glycolide) alone
摘要
Abstract
BACKGROUND: To mimic physiological and functional requirements of cartilage and subcondral bone, some recent studies have fabricated bilayered scaffolds for osteochondral tissue engineering in cartilage repair. However, in cartilage repair, and little is reported on the use of a single biomaterial instead of composite biomaterials to fabricate bilayered porous scaffolds. OBJECTIVE: To investigate the biocompatibility of an integrated bilayered porous scaffold fabricated with poly(lactic-co-glycolide) (PLGA), and then to discuss its feasibility of constructing tissue-engineered osteochondral graft. METHODS: The integrated bilayered porous scaffolds were fabricated with PLGA by changing the ultrastructure of scaffold.In vitro,bone marrow mesenchymal stem cells(BMSCs)isolated from rabbits were seeded into the bilayered porous PLGA scaffold, co-cultured for 1 week and then observed under scanning electron microscope. After co-culturing 48 hours,the cells were stained by LIVE/DEAD Kit.In vivo,the composite of DiI-labeled BMSCs and the scaffold or the scaffold alone were implanted subcutaneously into the skin of nude mice, and the implants were taken out and stained by hematoxylin-eosin and DAPI staining at 4 and 8 weeks after implantation. RESULTS AND CONCLUSION: (1) We successfully obtained the bilayered porous scaffolds with different pore sizes (the upper layer:100-200 μm and the lower layer:300-450 μm)and 85% porosity.(2)In the in vitro experiment,the LIVE/DEAD staining showed that BMSCs survived well in the scaffold, and the cells adhered well to the wall of pores in all the scaffolds and extracellular matrix deposition was found under the scanning electron microscope.(3)In the in vivo experiment, shown by the hematoxylin-eosin staining, a small amount of chondrocytes formed in the fibrous tissue in the upper layer and a large amount of trabecular bones in the lower layer were found at 8 weeks after implantation in the experimental group. The close integration between the upper layer and the lower layer appeared. BMSCs could survive for 8 weeks in vivo shown by the DAPI staining.However,in the control group,a little fibrous tissue was found without chondrocytes and apparent trabecular bone, and the scaffold was partially degraded. To conclude, the integrated bilayered porous scaffold fabricated with PLGA alone has good biocompatibility, which is feasibly used in osteochondral tissue engineering.关键词
生物材料/软骨组织工程/双层支架/PLGA/骨髓间充质干细胞/生物相容性/国家自然科学基金分类
医药卫生引用本文复制引用
段平国,郭润生,余兴元,潘震,李晓峰,李虎,刘俊,姚浩群..单一生物材料聚乳酸/羟基乙酸共聚物构建组织工程骨软骨的可行性[J].中国组织工程研究,2017,21(34):5423-5429,7.基金项目
国家自然科学基金项目(81401790) （81401790）
江西省自然科学青年基金重点项目(20171ACB21057) （20171ACB21057）
江西省自然科学基金项目(20151BAB205053,20161BAB205235) （20151BAB205053,20161BAB205235）
江西省教育厅科技项目重点项目(GJJ160028)the National Natural Science Foundation of China, No. 81401790 （GJJ160028）
the Natural Science Foundation for the Youth in Jiangxi Province, No. 20171ACB21057 （）
the Natural Science Foundation of Jiangxi Province, No.20151BAB205053, 20161BAB205235 （）
the Science and Technology Project of Jiangxi Provincial Education Department,No. GJJ160028 （）
