中国组织工程研究2017，Vol.21Issue(34)：5443-5448,6.DOI:10.3969/j.issn.2095-4344.2017.34.006
碳/碳复合材料表面掺镁羟基磷灰石生物涂层的体外性能
In vitro properties of magnesium doped hydroxyapatite coating on the surface of carbon/carbon composites
摘要
Abstract
BACKGROUND: In the early study, hydroxyapatite coatings containing magnesium (Mg-HA) were successfully prepared on the carbon/carbon composite surface by electromagnetic induction deposition. The effects of different induction deposition time on the morphology of Mg-HA coating were analyzed. OBJECTIVE:To investigate the in vitro properties of Mg-HA coating on the carbon/carbon composites. METHODS: HA (control group) and Mg-HA (experimental group) coating were prepared on the surface of carbon/carbon composites.(1)In vitro solubility:Carbon/carbon composites with different coatings were immersed in low-glucose DMEM medium, and surface morphology, ion concentration, and coating adhesion were tested before and after immersion. (2) Cytocompatibility: Carbon/carbon composites with different coatings were respectively cultured with mouse bone marrow mesenchymal stem cells for 6 days, and cell proliferation was measured by scanning electron microscope. RESULTS AND CONCLUSION: (1) One day after soaking, sediments formed on the coating surface in both groups, but there were more sediments in the experimental group than the control group. Six days after soaking, the sediments completely covered the coating surface in the experimental group, while there were still some gaps in the control group; Ca and P were both detected on the coating surface in the two groups, and the Mg content on the coating surface was obviously higher in the experimental group than the control group. (2) Six days after soaking, the concentrations of Ca, P and Mg in the control group continued to fall; in the experimental group, the concentrations of Ca and P were also reduced gradually, but the Mg concentration arose gradually. Moreover, the Mg concentration was significantly higher in the experimental group than the control group (P < 0.05). (3) The bonding strength of the coating in the experimental group was higher than that in the control group before and after 6 days soaking. In summary, the Mg-HA coating has better bonding strength and cell compatibility.关键词
生物材料/材料相容性/碳/碳复合材料/羟基磷灰石/涂层/结合力/国家自然科学基金分类
医药卫生引用本文复制引用
倪昕晔,熊信柏,尤瑞金,吴兴盛,李万帅..碳/碳复合材料表面掺镁羟基磷灰石生物涂层的体外性能[J].中国组织工程研究,2017,21(34):5443-5448,6.基金项目
国家自然科学基金资助(51672178) （51672178）
常州市社会发展项目(CJ20160029) （CJ20160029）
江苏省自然科学基金(BK20151181) （BK20151181）
常州市卫生高层次人才资助项目(2016CZLJ004)the National Natural Science Foundation of China, No. 51672178 （2016CZLJ004）
the Social Development Program of Changzhou City, No. CJ20160029 （）
the Natural Science Foundation of Jiangsu Province, No. BK20151181 （）
the High-Level Talent Fund of Changzhou Municipal Health Department, No. 2016CZLJ004 （）
