畜牧与兽医2017，Vol.49Issue(12)：70-74,5.
水貂TOll样受体5编码区基因的克隆及序列分析
Molecular cloning and sequence analysis of mink Toll-like receptor 5 gene CDS
摘要
Abstract
To investigate genetic revolution and structural characteristics of mink Toll-like receptor 5 (TLR5),RT-PCR was performed to amplify TLR5 gene fragments after total RNA was extracted from mink lung,and the whole coded sequence was obtained by splicing.The gene of 2577bp encodes 858 amino acid,in which 17.2％ Leucines and signal peptides of 20 amino acids are included.Protein prediction showed that the protein is hydrophilicity,consisting of extracellular,transmembrane and intracellular regions,which appears typical structural characteristics of TLR family.Sequence analysis indicated that TLR5 of mink has the highest homology with that of ferret (97％ identity),80％ higher homology with that of walrus,panda and polar bear,and almost 70％ homology with that of the other species.TLR5 is highly conservative during the evolution according to a phylogenetic tree.This study will provide a basis for investigation of TLR5 role in the immunologic mechanism and disease resistance in minks.关键词
水貂/Toll受体5/基因克隆/序列分析/结构预测Key words
mink/Toll-like receptor 5/gene cloning/sequence analysis/structural prediction分类
生物科学引用本文复制引用
姜合祥,蔡孜萌,秦晓冰,单虎..水貂TOll样受体5编码区基因的克隆及序列分析[J].畜牧与兽医,2017,49(12):70-74,5.基金项目
科技部科技基础性工作专项项目(2012FY111000) （2012FY111000）
山东省优秀中青年科学科研奖励基金(博士基金)(BS2011SW010) （博士基金）
山东省自主创新及成果转化专项(2014ZZCX07105) （2014ZZCX07105）
青岛农业大学研究生创新计划项目(1215004) （1215004）
