西南国防医药2017，Vol.27Issue(12)：1253-1256,4.DOI:10.3969/j.issn.1004-0188.2017.12.001
葛花提取液成分分析及其抑制小鼠体内酒精吸收实验
Component analysis of Pueravia Flowe Extract and experiment on inhibition of alcohol absorption in mice
摘要
Abstract
Objective To explore the main components of Pueravia Flowe extracted with two different solvents and the experiment effects in the inhibition of alcohol absorption in mice.Methods Pueravia Flowe was extracted with 95％ ethyl alcohol and water respectively.The contents of four main components (i.e.,total alkaloids,flavonoids,reducing sugar and protein) in the extract were measured.Healthy mice were randomly divided into a blank group,a model control group,a positive control group,a Pueravia Flowe ethanol extract (low,medium and high doses) group and a Pueravia Flowe water extract (low,medium and high doses) group (n=20,respectively).Each drug administration group was administrated with corresponding drug by preventive gavage.30 minutes later,each group was administrated with liquor by gavage.Afterwards,half of the mice in each group were taken to record the disappearance time of righting reflex.The eyeballs of the other half of the mice were removed to collect blood.Their livers were also removed.Gas chromatographic method was taken to determine the ethanol concentration in mice plasm and microplate reader was used to determine the activity of alcohol dehydrogenase (ADH) in livers.Results The contents of total alkaloids,flavonoids,reducing sugar and protein in Pueravia Flowe alcohol extract were 8.1,179.2,134.0 and 0.9 mg/g respectively and 6.0,66.2,226.0 and 93.9 mg/g respectively in water extract.The disappearance time of righting reflex in drug administration team was longer than that in the model group,the plasma ethanol concentration was lower than that in the model group,and the liver ADH activity was higher than that in the model group;the disappearance time of righting reflex in the mice in Pueravia Flowe ethanol extract with the same dosage was shorter than that in the water extraction group,the ethanol concentration was greater than that in the water extraction group,and the liver ADH activity was higher than that in the water extract group.Conclusion After preventive gavage of mice,Pueravia Flowe ethanol extract and water extract can prolong the disappearance time of righting reflex in mice in a certain concentration range,lower the blood ethanol concentration in mice,improve the liver ADH activity in mice and prevent alcoholism.关键词
葛花/提取液/成分/乙醇脱氢酶/翻正反射/小鼠Key words
Pueravia Flowe/Extract/component/ADH/righting reflex/mice分类
医药卫生引用本文复制引用
张翼,谭永红,袁吕江,于波涛..葛花提取液成分分析及其抑制小鼠体内酒精吸收实验[J].西南国防医药,2017,27(12):1253-1256,4.基金项目
四川省科技支撑计划项目(2014SZ0141) （2014SZ0141）
重庆市科委社会事业与民生保障科技创新专项(cstc2017shmsA10004) （cstc2017shmsA10004）
