西南林业大学学报2017，Vol.37Issue(6)：129-134,6.DOI:10.11929/j.issn.2095-1914.2017.06.021
11种植物提取物抗烟草花叶病毒活性研究
Anti-Tobacco Mosaic Virus Activity of 11 Plant Extracts
摘要
Abstract
In order to develop environmentally friendly antiviral agents from plants, the anti tobacco mosaic vi-rus ( TMV) activities of extracts from 11 plants were studied by half-leaf method. The results showed that petroleum ether extracts from Laggera pterodonta, the ethyl acetate extracts from Oenothera rosea and the water extracts from L. pterodonta showed potent antiviral proliferation activities with inhibition rates of 68. 47%, 80. 19% and 94. 57%respectively at the concentration of 1 mg/mL. The petroleum ether extracts from L. pterodonta, ethyl acetate extracts from Aster subulatus and water extracts from L. pterodonta showed prominent inactivation activities against TMV with inhibition rate 80. 00%, 76. 38% and 83. 33%, respectively. According to TAS-ELISA experiment, extracts from L. pterodonta, Clinopodium polycephalum, Solanum pseudo-capsicum, A. subulatus, Leonurus Artemisia, Sida szechuensis showed significant anti-TMV proliferation activities in Nicotiana tabacum CV. K326. The antivirus components from these plants were worth further investigation.关键词
烟草花叶病毒/抗TMV活性/半叶枯斑法/叶圆片法/检测Key words
tobacco mosaic virus ( TMV)/anti-TMV activity/half-leaf method/TAS-ELISA/detection分类
农业科技引用本文复制引用
祁进康,邹正彪,王德艳,毕再涛,钱瑞,陈永对,闫晓慧..11种植物提取物抗烟草花叶病毒活性研究[J].西南林业大学学报,2017,37(6):129-134,6.基金项目
国家自然科学基金项目(31200265、31560106)资助 （31200265、31560106）
西南林业大学博士科研启动经费(111712)资助 （111712）
云南省高校优势特色重点学科(生物学)建设项目资助 （生物学）
云南省林学一流学科建设经费资助 （）
云南省高校森林有害生物科技创新团队项目资助 （）
云南省森林灾害预警与控制重点实验室开放基金项目(ZK150009)资助. （ZK150009）
