中国临床药理学与治疗学2017，Vol.22Issue(10)：1117-1122,6.
奥巴克拉联合5-氟尿嘧啶对低氧性胰腺癌细胞上皮间质转化的影响
Effects of obatoclax plus 5-Fluorouracil on the epithelial mesenchymal transition of pancreatic cancer cells under hypoxia
摘要
Abstract
AIM:To explore the effect of obatoclax combined with 5-Fluorouracil (5-FU) in epithelial mesenchymal transition (EMT) of pancreatic cancer BxPC-3 cells and its possible mechanism.METHODS:MTT assay was performed to detect cell viability in hypoxic group,5-FU group and obatoclax combined with 5-FU group.RT-qPCR and Western blotting were used to detect EMT-related genes and proteins expression in BxPC-3 cells of hypoxic group,5-FU group and obatoclax combined with 5-FU group.RESULTS:Under hypoxia condition,obatoclax combined with 5-FU time-dependently inhibited BxPC-3 cell proliferation activity.Obatoclax combined with 5-FU further down-regulated the expression of transcription factors Snail and Slug,as well as mesenchymal markers N-Cadherin,Fibronectin,Collagen Ⅰ and Vimentin.Obatoclax combined with 5-FU up-regulated the expression of epithelial marker E-Cadherin.CONCLUSION:Under hypoxia condition,obatoclax combined with 5-FU inhibit the viability of BxPC-3 cells.Obatoclax combined with 5-FU can up-regulate E-Cadherin and down-regulate N-Cadherin,Fibronectin,Collagen Ⅰ and Vimentin through inhibiting Snail and Slug,thus inhibiting EMT,reducing migration and invasion ability of BxPC-3 cells.关键词
上皮间质转化/低氧/奥巴克拉/5-氟尿嘧啶/胰腺癌Key words
epithelial mesenchymal transition/hypoxia/obatoclax/5-Fluorouracil/pancreatic cancer分类
医药卫生引用本文复制引用
侯新芳,宋海岩,周志新,邓晓慧,张毅敏,程慧欣..奥巴克拉联合5-氟尿嘧啶对低氧性胰腺癌细胞上皮间质转化的影响[J].中国临床药理学与治疗学,2017,22(10):1117-1122,6.基金项目
河南省医学科技攻关计划项目(201702240) （201702240）
新乡医学院科研培育基金(2014QN119) （2014QN119）
