中国临床药理学与治疗学2017，Vol.22Issue(10)：1133-1137,5.
UPLC-MS/MS法测定卡泊芬净血浆浓度
Determination of caspofungin in human plasma by UPLC-MS/MS
摘要
Abstract
AIM:To establish a method to determine the concentration of caspofungin in human plasma by UPLC-MS/MS.METHODS:Plasma samples were spiked with roxioxithromycin as the internal standard and precipitated by methanol.The column was Ultimate AQ C18 (100 mm × 2.1 mm,3 μm) with the column temperature of 40 ℃.The mobile phase was acetonitrile and aqueous solution (5 mmol/L ammonium acetate,0.2％ formic acid),and the pump flow rate was 0.4 mL/min.The ion transitions were performed under ESI positive MRM model at m/z 547.5→538.2 for caspofungin and m/z 838.6→157.9 for internal standard.The analysis time was 6.5 min.RESULTS:Calibration curves of caspofungin showed good linear regression in the range of 0.1-25 μg/mL (R2 =0.999 5).The lowest quantification limit of caspofungin was 0.1 μg/mL.The extraction recovery was about 59.08％.The intra-day and inter-day relative standard deviation (RSD) were less than 15％.CONCLUSION:The established method is simple,accurate,sensitive and had good reproducibility,which can be used for the blood concentration determination of caspofungin.关键词
卡泊芬净/UPLC-MS/MS法/治疗药物监测Key words
caspofungin/UPLC-MS/MS/therapeutics drug monitoring分类
医药卫生引用本文复制引用
邵华,何子剑,胡琳磷,詹莹,胡蓉蓉,何杰..UPLC-MS/MS法测定卡泊芬净血浆浓度[J].中国临床药理学与治疗学,2017,22(10):1133-1137,5.基金项目
江苏省自然科学基金(BK20150645) （BK20150645）
