中国临床药理学与治疗学2017，Vol.22Issue(11)：1215-1220,6.
白藜芦醇通过高迁移率族蛋白B1抗大鼠肝脏缺血再灌注损伤的作用及机制
Effect and mechanism of resveratrol alleviates hepatic ischemia reperfusion injury by HMGB1 in rats
摘要
Abstract
AIM:To investigate the effect and mechanism of resveratrol on the expression of high mobility group box 1 in rats with hepatic ischemiareperfusion injury.METHODS:Thirty healthy male Sprague-Dawley (SD) rats were randomly divided into three groups:sham control (SC) group,hepatic ischemia reperfusion injury (HIR) group and resveratrol (Res) group.The blood samples and liver tissue samples were collected.The pathological changes of the liver were observed.Serum ALT,AST and AKP activity were detected as indicators of liver function damage and the content of TNF-α and IL-6 in serum were detected by ELISA.The expressions of HMGB1 and TLR4 protein and mRNA were determined by Western Blot and RTPCR.RESULTS:Compared with the HIR group,the pathological injury of the liver was significantly improved and the concentrations of serum AST,ALT and AKP were significantly lower in Res group (P ＜0.01);meanwhile,the expression of HMGB1 and TLR4 protein and mRNA of hepatic tissue were decreased,serum inflammatory factors IL-6 and TNF-α were also significantly decreased (P ＜ 0.01).CONCLUSION:Resveratrol shows significant protective effect on hepatic ischemia reperfusion injury in rat.This may be related with inhibiting the HMGB1-TLR4 signaling pathway and alleviating the inflammatory response.关键词
肝脏缺血再灌注损伤/高迁移率族蛋白B1/Toll样受体4/白藜芦醇/炎症因子Key words
hepatic ischemia reperfusion injury/high mobility group box 1/Toll-like receptor 4/resveratrol/inflammatory cytokine分类
医药卫生引用本文复制引用
宋娟,田小霞,李宝红,王东,孟董超,卢彦珍..白藜芦醇通过高迁移率族蛋白B1抗大鼠肝脏缺血再灌注损伤的作用及机制[J].中国临床药理学与治疗学,2017,22(11):1215-1220,6.基金项目
国家自然科学基金资助项目(81070339) （81070339）
长治医学院科技启动基金资助项目(QDZ201520) （QDZ201520）
长治医学院大学生创新创业训练计划项目(D2016001) （D2016001）
