中国临床药理学与治疗学2017，Vol.22Issue(11)：1237-1243,7.
桂枝-白芍药对不同比例配伍对芍药苷在大鼠体内药动学的影响
Effect of different proportions of Ramulus Cinnamomi and Radix Paeoniae Alba on the pharmacokinetics of paeoniflorin in rats
摘要
Abstract
AIM:To develop and validate a simple and sensitive high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the quantification of paeoniflorin and to investigate the effect of different proportions of Ramulus Cinnamomi and Radix Paeoniae Alba on the pharmacokinetics of paeoniflorin in rats.METHODS:After the different proportion of Ramulus Cinnamomi and Radix Paeoniae Alba were orally administrated to each group at a paeoniflorin dose of 50 mg/kg in rats,the plasma samples were collected at the designated time points.The plasma concentration of paeoniflorin was determined by HPLC-MS/MS,warfarin as the internal standard.The main pharmacokinetic parameters were calculated and analyzed.RESULTS:The results of HPLCMS/MS assay in plasma samples were in accordance with the requirements of biological samples.This method was successfully applied to the pharmacokinetics study of paeoniflorin in rats.The ratio of Ramulus Cinnamomi and Radix Paeoniae Albaat 1:1 showed the rapid drug absorption trends.The C AUC(0-1) and AUC(0-∞) of single peony group was statistically significant compared with other groups (P ＜ 0.05 or P ＜ 0.01).The relative bioavailability of paeoniflorin in different proportions of Persicae Ramulus and Paeoniae Radix Alba were 302.61％,155.56％,245.45％,respectively.There was no significant change in tmax,but there was significant difference in biological half-life (P ＜ 0.05)between each group.CONCLUSION:The pharmacokinetics of paeoniflorin was affected by the proportion of Ramulus Cinnamomi and RadixPaeoniae Alba.In the view of pharmacokinetics,this experiment explains the scientific nature and rationality of ancient traditional Chinese to a certain extent.关键词
桂枝/白芍/配伍/药动学/芍药苷Key words
Ramulus Cinnamomi/Radix Paeoniae Alba/compatibility/pharmacokinetics/paeoniflorin分类
医药卫生引用本文复制引用
陈永财,钱江辉,王彬辉,沙先谊,翁金月..桂枝-白芍药对不同比例配伍对芍药苷在大鼠体内药动学的影响[J].中国临床药理学与治疗学,2017,22(11):1237-1243,7.基金项目
浙江省中医药资助项目(2015ZA195) （2015ZA195）
温州市公益性科技计划项目(Y20140746) （Y20140746）
