中国临床药理学与治疗学2017，Vol.22Issue(11)：1244-1247,1303,5.
丹酚酸A抗肿瘤作用及对逆转A549/MTX肿瘤多药耐药的影响
Antitumor effect of salvianolic acid A and on its reversal of multidrug resisitance in A549/MTX tumor
摘要
Abstract
AIM:To explore the anti-tumor effect of salvianolic acid A(SAA) and to research its reversal effect of multidrug resistance on A549/MTX tumor.METHODS:The human lung carcinoma cell line A549 and human lung carcinoma drug-resisitant cell line A549/MTX were cultured in vitro and were divided into three groups:blank control group (medium only),negative control group (cell suspension plus medium) and experimental group (cell suspension plus three kinds of ant-tumor drugs).SAA in five concentrations (4,8,16,32,and 64 μg/mL) intervened A549 cells.The inhibition effects of SAA of 4,8,16,32,and 64 μg/mL in 24 h,48 h and 48 h on A549 cell proliferation were tested by MTT assay.The IC50 of MTX,CDDP,and GEF for the cell of A549,A549/MTX were determined by MTT assay.The IC50 of MTX,CDDP,and GEF for the A549/MTX after intervention with SAA were determined also by MTT assay.The sensitivity change and drug reversal effect of SAA before and after intervention on the A549/MTX cell line were investigated by MTT assay.RESULTS:The inhibition rate after intervention with SAA on A549 cells for 24 h was increased from (9.16 ± 3.64)％ (4 μg/mL) to (52.93 ±5.21)％ (64 μg/mL).Accordingly,the inhibition rate of SAA were (54.93 ±5.21)％,(63.83 ±2.74)％,(72.91 ±4.06) ％ for 24 h,48 h and 72 h as the concentration was increased to 64 μg/mL.Anti-proliferative effects of SAA on A549 cells were significant,and the inhibition rate increased with dose dependence and time dependence.Inhibition rate of SAA of 4 μg/mL on A549 and A549/MTX cell growth were 9.16％ and 7.38％,respectively.Both less than 10％ and no obvious toxic effect was observed,so concentration of 4 μg/mL was choosed as safety experiment dose for lung cancer drug resistant.When 4 μg/mL SAA intervened on A549/MTX cell,MTX to A549/MTX half inhibitory concentration was from (105.72±4.62) μg/mLto (26.13 ±1.36) μg/mL.Reverse ratio was 4.05.Half inhibitory concentration of CDDP on A549/MTX cell was from (174.92 ±6.86) μg/mL to (49.89 ±1.73) μg/mL,reverse ratio was 3.51.Half inhibitory concentration of GEF on A549/MTX cell was from (251.38 ±8.64) μg/mL to (116.93 ±5.22) μg/mL,reverse ratio was 2.15.Compared with the effect on A549 cells,MTX,CDDP,and GEF presented significant difference (P ＜ 0.01).Compared with the effect on A549/MTX cell intervened with SAA,MTX,CDDP,and GEF presented significant effect on A549/MTX cells (P ＜0.01).CONCLUSION:SAA can inhibit the growth of A549 cells and A549/MTX cells,and partially reverse the multidrug resistance of A549/MTX in vitro.关键词
丹酚酸A/抗肿瘤/多药耐药/半数抑制浓度Key words
salvianolic acid A (SAA)/antitumor/multi-resistant/half maximal inhibitory concentration分类
医药卫生引用本文复制引用
李晖,陈俊,徐彩虹,庞林荣,程晓春..丹酚酸A抗肿瘤作用及对逆转A549/MTX肿瘤多药耐药的影响[J].中国临床药理学与治疗学,2017,22(11):1244-1247,1303,5.基金项目
浙江省中医药科学研究基金项目(2011ZB135) （2011ZB135）
