中国临床药理学杂志2017，Vol.33Issue(21)：2154-2157,4.DOI:10.13699/j.cnki.1001-6821.2017.21.018
酒精性肝损伤小鼠在损伤过程中细胞凋亡时间点研究
Study on the time of cell apoptosis in alcoholic liver injury in mice
摘要
Abstract
Objective To study the changes of hepatocyte apoptosis during alcoholic liver injury in mice.Methods Ninety healthy male KM mice were randomly separated into control group (n =10),model group (n =80).The model group were given 56 ° ethanol (7.5 μL · g-1 · d-1)by gavage for eight weeks,ten of which were dislocated executed at 1,2,3,4,5,6,7,8 weeks,respectively.After the intragastric administration,to take the liver tissue.The mice in the control group were sacrificed after the same amount of distilled water,and the liver was tested and analyzed.Changes of cell apoptosis of alcoholic-incuded liver injury were detected by Hematoxylin and eosin (HE) and Hoechst33258 staining.To identify and count of apoptotic cells and normal cells under a fluorescence microscope,which can be used to calculate the rate of apoptosis.Results Acute hepatic injury were observed at third week,manifested as increased inflammatory cell infiltration.The eighth week showed chronic liver damage,characterized by degeneration of liver tissue water and fat vacuoles.The apoptotic rates of the model group were (9.28 ±0.41)％,(13.20 ±0.81)％ and (20.43 ±0.97)％,at 1-3 weeks,respectively.Compared with the control group (1.38 ±0.18)％,hepatocyte apoptosis was significantly increased at three weeks (all P ＜0.05).Liver cell apoptosis rate increased gradually,with the extension of the time.Apoptosis rate reached (51.99 ± 1.70) ％ at eight weeks,which was significantly different from that of the control group (P ＜ 0.01).Conclusion The degree of liver injury induced by alcohol is closely related to the development of liver cell apoptosis.关键词
酒精性肝损伤/Hoechst33258染色/苏木素-伊红染色/细胞凋亡Key words
alcoholic liver injury/Hoechst33258 staining/Hematoxylin and eosin staining/cell apoptosis分类
医药卫生引用本文复制引用
李三强,卢华杰,王萍,王冬梅,王善龙,张勇勇,宋影..酒精性肝损伤小鼠在损伤过程中细胞凋亡时间点研究[J].中国临床药理学杂志,2017,33(21):2154-2157,4.基金项目
国家自然科学基金资助项目(81201558) （81201558）
河南省高校科技创新团队基金资助项目(181RTSTHN026) （181RTSTHN026）
河南省科技攻关基金资助项目(172102310693) （172102310693）
河南科技大学创新团队基金资助项目(2015XTD003) （2015XTD003）
