中国农业科学2017，Vol.50Issue(22)：4362-4372,11.DOI:10.3864/j.issn.0578-1752.2017.22.012
宽皮柑橘种质资源表型多样性分析及综合评价
Comprehensive Evaluation and Phenotypic Diversity Analysis of Germplasm Resources in Mandarin
摘要
Abstract
[Objective] The objective of this study is to evaluate some major phenotypic characteristics diversity of mandarin germplasms and explore the comprehensive method for screening important mandarin germplasm resources.[Method] The diversity evaluation of 18 phenotypic traits in 239 mandarin accessions were carried out.Variation coefficients,Shannon-Weaver Information index cluster analysis,principal component analysis,correlation and regression analysis were comprehensively used to evaluate the relationship of characteristics and select important characteristics.[Result] Based on the variation coefficient of 239 mandarins,large variations were found in the traits of seed numbers per fruit (SNF),the ratio of soluble solids to titratable acidity (TSS/TA),the contents of titratable acid (TA),fruit weight (FW),while genetic characteristics of fruit shape index(FSI),segment numbers per fruit (SNPF),the contents of total soluble solids (TSS) kept relatively stable.Shannon-Weaver Information index showed that the diversity level of FW,fruit diameter (FD),fruit length (FL),SNPF,petiole length (PL),leaf lamina length (LLL),leaf lamina width (LLW),the contents of reducing sugar (RS) and invert sugar (IS) were high,while fruit surface texture(FST),SNF,TSS/TA had low diversity level.The variance analysis was conducted to evaluate the trait difference of 239 mandarin accessions,which divided into five different geographical origins.The result showed that mandarin accessions from America have higher average contents of TSS and IS;the mandarin accessions from Japan have larger FW,FD and FL and lower SNF.Mandarin accessions from the Yangtze River Basin were larger fruit size,higher contents of the TSS,RS and IS than the Pearl River Basin,but lower TSS/TA.Variance analysis for wild germplasms,landraces and bred varieties showed that bred accessions have larger fruits,smoother peels,fewer seeds,lower contents of TA and higher contents of sugar content.Both the cluster analysis and PCA suggested genetic variations were presented in different geographical origins.Small fruit type accession from China should be thought as an unique subgroup,Green Peel Mi Ju is an unique citrus accession.Principal component analysis showed that the variation cumulative contribution rate of the first nine principal components was accounted for 81.94％.The average F value from the comprehensive evaluation of phenotypic traits of 239 mandarins was 0.480.The highest F value (0.664) was given to Ehime Kashi No.21 from Japan.The lowest F value (0.211) was Shikuwasha.The correlation analysis between the phenotypic traits and F value showed that the phenotypic traits except FST were significantly correlated with the F value.Nine phenotypic traits,including fruit length (FL),FSI,FST,SNPF,PL,LLW,the contents of TSS,TA and RS,were selected out as evaluation indexes by stepwise regression analysis.[Conclusion] Mandarin accessions presented large phenotypic diversity.The traits difference of the accessions from different geographical origins or germplasm types are great.Nine phenotypic traits could be used as important evaluation charaterisitics for screening unique mandarin.关键词
宽皮柑橘/表型多样性/不同地理来源/综合评价Key words
mandarin/phenotypic diversity/different geographical origins/comprehensive evaluation引用本文复制引用
孙珍珠,李秋月,王小柯,赵婉彤,薛杨,冯锦英,刘小丰,刘梦雨,江东..宽皮柑橘种质资源表型多样性分析及综合评价[J].中国农业科学,2017,50(22):4362-4372,11.基金项目
国家“十二五”科技支撑计划(2013BAD01B04)、重庆市科委重点项目(cstc2016shms-ztzx80004) （2013BAD01B04）
