中国现代医学杂志2018，Vol.28Issue(2)：14-19,6.DOI:10.3969/j.issn.1005-8982.2018.02.003
PRF对下颌骨牵引成骨区骨保护素表达的影响
Effect of platelet-rich fibrin on distraction osteogenesis and osteoprotegerin expression
摘要
Abstract
Objective To investigate the effect of platelet-rich fibrin (PRF) on mandibular osteogenesis and the expression of osteoprotegerin (OPG) in the period of distraction osteogenesis. Methods Twenty-five mature rabbits were randomly divided into five groups, bilateral mandibular osteotomies were performed in the rabbits. PRF was implanted in one side of the mandible as experimental group. The other side of madible was taken as control group. The rabbits were sacrificed and the distracted calluses were harvested and processed for HE and OPG immunohistochemical staining on the 1st, 3th, 7th, 14th and 28th day after the end of distraction. The expression of OPG in the calluses was analyzed by cell digital imaging soft-ware. Results The regenerated bone was found in the distraction gap after mandibular lengthening, OPG was co-localized in cytoplasm of osteoblasts and newly-embedded osteocytes. Compared with the control group, the OPG-positive cell number was statistically different in the experimental group on the 1st, 3th, 7th, 14th and 28th day after distraction (P < 0.05). Conclusions Our preliminary result demonstrated that PRF increases new bone formation and has a positive effect on early bone healing. OPG may play important roles at the early stage of mandibular distraction.关键词
下颌骨/牵引成骨/新骨形成/富血小板纤维蛋白/骨保护素Key words
mandible/distraction osteogenesis/new bone formation/platelet-rich fibrin/osteoprotegerin分类
医药卫生引用本文复制引用
付颖,董庆文,李新,王稚英,许王汉..PRF对下颌骨牵引成骨区骨保护素表达的影响[J].中国现代医学杂志,2018,28(2):14-19,6.基金项目
辽宁省自然科学基金(No:2015020352) （No:2015020352）
辽宁省大学生创新创业训练计划项目(No:201510160000019) （No:201510160000019）
