中国现代医学杂志2018，Vol.28Issue(2)：20-25,6.DOI:10.3969/j.issn.1005-8982.2018.02.004
PEDF、END在UUO肾组织的表达及ADSCs对其的影响
Expressions of PEDF and endostatin in rat kidney after unilateral ureteral obstruction and effect of adipose-derived stem cells on them
摘要
Abstract
Objective To observe the dynamic changes of pigment epithelium-derived factor (PEDF) and endostatin (END) expressions in renal tissues 3, 7 and 14 days after unilateral ureteral obstruction (UUO) in rats and the expression changes after the intervention of adipose-derived stem cells, so as to explore the possible mechanism of PEDF and END in UUO and the relationship between them and the effect of adipose-derived stem cells on the expressions of PEDF and END in the course of UUO. Methods Seventy healthy adult male SD rats were randomly divided into sham operation control group (10 rats), UUO group (30 rats) and intervention group (30 rats). The UUO model was produced by unilateral ureteral obstruction and the rats in the intervention group were injected with adipose-derived stem cells in 24 hours. On the 3rd, 7th and 14th day after ureteral obstruction, the progression of renal injury and fibrosis and the expressions of PEDF and END in rats were observed. Results The renal injury and fibrosis got more severe with the prolongation of the time of obstruction, the expressions of PEDF and END in the UUO group were higher than those of the control group at each time point. The expressions of END and PEDF were decreased at all time points after intervention by adipose-derived stem cells. Conclusions The upregulation of PEDF and END after UUO may be one of the important mechanisms of microvascular occlusion mediated by regulation abnormality of microvascular injury and angiogenesis. Intervention of adipose-derived stem cells can promote the repair of vascular injury and angiogenesis by inhibiting the expressions of PEDF and END.关键词
色素上皮衍生因子/内皮抑素/表达/脂肪干细胞/影响Key words
pigment epithelium-derived factor/endostatin/expression/adipose-derived stem cell/effect分类
医药卫生引用本文复制引用
吕春燕,杨天林,李进辉,吴程,王丽,陈昌金,赵梓亦..PEDF、END在UUO肾组织的表达及ADSCs对其的影响[J].中国现代医学杂志,2018,28(2):20-25,6.基金项目
四川省卫生厅项目(No:110520) （No:110520）
四川省成都市卫生局项目(No:2014004) （No:2014004）
