中国现代医学杂志2018，Vol.28Issue(2)：33-36,4.DOI:10.3969/j.issn.1005-8982.2018.02.006
丙泊酚对小胶质细胞炎症因子的影响及其机制研究
Effect of Propofol on inflammatory cytokines in microglia and its mechanism
摘要
Abstract
Objective To investigate the effect of Propofol on inflammatory cytokines in microglia and its mechanism. Methods Microglial BV-2 cells were divided into control group, Propofol group, lipopolysaccharide (LPS) group, and LPS+Propofol group. The cells of the control group were added with PBS. The cells of the Propofol group were added with 30 μmol/L Propofol. The cells of the LPS group were treated with 1 μg/ml LPS. The cells of the LPS+Propofol group were added with 30 μmol/L Propofol and 1 μg/ml LPS. The cell viability was determined by MTT colorimetric assay. The levels of interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in supernatant were measured by ELISA. The expressions of p38MAPK and TLR4 mRNAs were detected by RT-PCR. The expressions of p38MAPK and TLR4 proteins were detected by Western blot. Results The activity of microglia in the LPS group and the LPS+Propofol group were lower than that in the control group and the Propofol group (P < 0.05). The activity of microglia in the LPS+Propofol group was higher than that in the LPS group (P < 0.05). The levels of IL-1β, IL-6 and TNF-α in the supernatant of the LPS group and the LPS+Propofol group were higher than those in the control group and the Propofol group (P < 0.05). The levels of IL-1β, IL-6 and TNF-α in the supernatant of the LPS+Propofol group were lower than those in the LPS group (P < 0.05). The mRNA and protein expressions of p38MAPK and TLR4 in the LPS group and the LPS+Propofol group were higher than those in the control group and the Propofol group (P < 0.05). The mRNA and protein expressions of p38MAPK and TLR4 in the LPS+Propofol group were lower than those in the LPS group (P < 0.05). There were no significant differences in the above indices between the Propofol group and the control group (P > 0.05). Conclusions Propofol has the effect of inhibiting the hyperactivity and inflammatory response of microglia, and its mechanism may be related to the down-regulation of TLR4-p38MAPK signaling pathway.关键词
丙泊酚/小胶质细胞/炎症因子Key words
Propofol/microglia/inflammatory factor分类
医药卫生引用本文复制引用
叶雪飞,左春龙,梅虹霞,苏颖,杨建平..丙泊酚对小胶质细胞炎症因子的影响及其机制研究[J].中国现代医学杂志,2018,28(2):33-36,4.基金项目
浙江省医药卫生科技计划项目(No:2016KYA140) （No:2016KYA140）
