中国烟草学报2017，Vol.23Issue(6)：88-93,6.DOI:10.16472/j.chinatobacco.2017.073
一种蚜茧蜂基因组DNA提取方法
Method for extraction of genomic DNA from Aphidiidae
摘要
Abstract
In order to eff ectively extract genomic DNA of Aphidiidae and reserve specimens for morphological identifi cation, extraction method of conventional kits (column centrifugation method) was improved. Genomic DNA of 7 species of Aphidiidae was extracted by puncturing,and genomic DNA of 4 species among them were extracted by grinding. Purity and yield of genomic DNA extracted by two methods were compared and verifi ed by amplifi cation of m itochondrial COI gene sequences. Results showed that puncturing extraction could be used to extract genomic DNA from individual adult of Ahidiidae, and to reserve specimens for morphological identifi cation. The yield of DNA extracted by this method ranged from 29 to 55 ng/μL,OD260 /OD280 of the extracts ranged from 1.51 to 1.91, which could stably amplify mitochondrial COI gene sequences, and PCR amplifi ed bands were clear and complete.关键词
蚜茧蜂/基因组DNA/DNA提取/PCRKey words
Aphidiidae/genomic DNA/DNA extraction/PCR引用本文复制引用
孔博,张宏瑞,和淑琪,谷星慧,杨硕媛,张立猛,李正跃..一种蚜茧蜂基因组DNA提取方法[J].中国烟草学报,2017,23(6):88-93,6.基金项目
云南农业大学研究生科技创新项目(2016ykc16),中国烟草总公司科技重大专项项目(110201601021) （2016ykc16）
