中国烟草学报2017,Vol.23Issue(6):94-98,5.DOI:10.16472/j.chinatobacco.2017.075
新鉴定高抗TMV普通烟草枯斑资源的N导入片段长度多样性
N introgression segment length polymorphism of newly testedNicotiana tabacum germplasm highly resistant to tobacco mosaic virus
摘要
Abstract
In order to understand the status ofNicotiana tabacum germplasm highly resistant to tobacco mosaic virus (TMV), to provide information for highly resistant parents selection in tobacco breeding, tobacco germplasm with N gene were selected by using N gene-specifi c molecular markers, and their N introgression segment lengths were tested by using N introgression segment specifi c molecular markers. Results showed that 63 tested tobacco germplasm could be divided into three main categories, Coker176 type: GL3.50 and GL4.06 marker negative, TN4.39, TN5.226, TN5.51 marker positive; Samsun NN type: GL3.50, GL4.06, TN4.39, TN5.226 marker positive, TN5.51 marker negative; Xanthi NC type: GL3.50, GL4.06, TN4.39, TN5.226, TN5.51 marker positive. The N introgression segment length of 402 and other 51 germplasm belonged to Coker176 type. The N introgression segment length of Ky160 and other 1 germplasm belonged to Samsun NN type. The N introgression segment length of Ky52 and other 9 germplasms belonged to Xanthi NC type. Germplasm with short N introgression segment may have less linkage drag, and are of greater breeding potential. The N introgression segment lengths of TMV highly resistant germplasm were evaluated in this paper, which could provide information for selecting highly resistant parents in variety development.关键词
普通烟草/N导入片段/长度Key words
Nicotiana tabacum/N introgression segment/length引用本文复制引用
刘勇,李梅云,于海芹,黄昌军,李永平..新鉴定高抗TMV普通烟草枯斑资源的N导入片段长度多样性[J].中国烟草学报,2017,23(6):94-98,5.基金项目
中国烟草总公司云南省公司科技项目(2014YN02),中国烟草总公司科技项目(110201401001) (2014YN02)