中国中医药信息杂志2018，Vol.25Issue(1)：64-68,5.DOI:10.3969/j.issn.1005-5304.2018.01.014
响应面法优选银槐解毒颗粒提取工艺
Optimization of Extraction Process of Yinhuai Jiedu Granula by Response Surface Methodology
摘要
Abstract
Objective To optimize extraction process of Yinhuai Jiedu Granula.Methods On the basis of single factor experiments, effects of soaking time, solvent dosage and extracting time on contents of chlorogenic acid and total flavonoids were investigated by response surface methodology. HPLC was used to determine contents of chlorogenie acid and total flavonoids with mobile phase of acetonitrile-0.1% phosphoric acid for gradient elution (0–20 min, 13%A; 20–30 min, 13%–18%A; 30–55 min, 18%–20%A; 55–65 min, 20%–23%A) and detection wavelengthes of 327 nm and 256 nm for chlorogenic acid and total flavonoids respectively. Results Optimal extraction process was as following: soaked 60 minutes and extracted twice with 12 times the amount of water per time of 130 minutes. Concentrations of chlorogenic acid, rutin and baicalin were 51.497, 68.872, 25.763 μg/g respectively. The average extract yield was 33.105%. Conclusion The optimized extraction process is feasible and stable,which can provide data support for the further research on Yinhuaijiedu granula.关键词
银槐解毒颗粒/绿原酸/芦丁/黄芩苷/响应面分析法Key words
Yinhuai Jiedu Granula/chlorogenic acid/rutin/baicalin/response surface分类
医药卫生引用本文复制引用
张江,张丽,木卡代斯·斯依提,赵翡翠..响应面法优选银槐解毒颗粒提取工艺[J].中国中医药信息杂志,2018,25(1):64-68,5.基金项目
乌鲁木齐市科学技术计划(Y151310012) （Y151310012）
